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Volume 66, Issue 3

sp. nov., an endophytic actinobacterium isolated from root of Free

Abstract

Two strains of Gram-stain-positive, aerobic, non-motile, irregular coccus-shaped bacteria, designated YIM 2617 and YIM 2617-2, were isolated from the root of . Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the two strains were closely related to YIM M13146 (98.1 and 98.0 % similarities, respectively) and formed a robust clade with in the neighbour-joining tree. Optimum growth of the strains was observed at 28–32 °C, at pH 7.0–8.0 and in the presence of 0–2 % (w/v) NaCl. The chemotaxonomic profiles of the strains comprised anteiso-C as the major cellular fatty acid, MK-9(H) as the predominant menaquinone, alanine, glycine, glutamic acid and -diaminopimelic acid as peptidoglycan components, and phosphatidylcholine and diphosphatidylglycerol as the major polar lipids. The G+C contents of the genomic DNA of strains YIM 2617 and YIM 2617-2 were 66.4 and 66.6 mol%, respectively. DNA–DNA hybridizations of the two strains with YIM M13146 gave mean relatedness values of 47.6 ± 2.0 and 43.2 ± 1.5 %, respectively, while the relatedness value between them was 92.1 ± 2.2 %. On the basis of the data recorded from the present study, strains YIM 2617 and YIM 2617-2 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 2617 ( = KCTC 29482 = DSM 28728 = JCM 30097).

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2016-03-01
2024-04-19
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Mariniluteicoccus endophyticus sp. nov., an endophytic actinobacterium isolated from root of Ocimum basilicum
Int J Syst Evol Microbiol 66, 1306 (2016); https://doi.org/10.1099/ijsem.0.000880
/content/journal/ijsem/10.1099/ijsem.0.000880
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