1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 3

sp. nov., isolated from estuary sediment Free

Abstract

A Gram-stain-negative, strictly aerobic, non-motile bacterium, designated strain S2-C, was isolated from an estuary sediment in South Korea and subjected to a polyphasic taxonomic investigation. Cells were catalase- and oxidase-positive rods without gliding motility. Growth of strain S2-C was observed at 15–40 °C (optimum, 30 °C), at pH 5.5–9.0 (optimum, pH 6.5–7.5) and in the presence of 0–7.0 % (w/v) NaCl (optimum, 0–2 %). Only ubiquinone-8 (Q-8) was detected as the isoprenoid quinone and iso-C, iso-C, summed feature 9 (comprising iso-Cω9 and/or C 10-methyl), iso-C, iso-C 3-OH and iso-C were found to be the major cellular fatty acids. Strain S2-C contained phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. The G+C content of the genomic DNA was 63.8 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain S2-C formed a distinct phyletic lineage within the genus . Strain S2-C was most closely related to GH1-9 with 97.1 % 16S rRNA gene sequence similarity and the DNA–DNA relatedness value between strain S2-C and the type strain of was 45.7 ± 2.2 %. On the basis of the phenotypic, chemotaxonomic and molecular features, strain S2-C clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S2-C ( = KACC 18502 = JCM 31130).

  • Published Online:
© 2016 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000884
2016-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/3/1346.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000884&mimeType=html&fmt=ahah

References

  1. Bae H.-S., Im W.-T., Lee S.-T. 2005; Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 55:1155–1161 [View Article][PubMed]
     [Google Scholar]
  2. Christensen C., Cook F. D. 1978; Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. Int J Syst Bacteriol 28:367–393 [CrossRef]
     [Google Scholar]
  3. Felsenstein J. 2002; phylip (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  4. Fukuda W., Kimura T., Araki S., Miyoshi Y., Atomi H., Imanaka T. 2013; Lysobacter oligotrophicus sp. nov., isolated from an Antarctic freshwater lake in Antarctica. Int J Syst Evol Microbiol 63:3313–3318 [View Article][PubMed]
     [Google Scholar]
  5. Gomori G. 1955; Preparation of buffers for use in enzyme studies. In Methods in Enzymology vol. 1 pp 138–146Edited by Colowick S. P., Kaplan N. O. New York: Academic press; [View Article]
     [Google Scholar]
  6. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [View Article][PubMed]
     [Google Scholar]
  7. Jeong S. H., Jin H. M., Lee H. J., Jeon C. O. 2013; Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 63:971–976 [View Article][PubMed]
     [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  9. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  10. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
     [Google Scholar]
  11. Lee S. H., Shim J. K., Kim J. M., Choi H.-K., Jeon C. O. 2011; Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . Int J Syst Evol Microbiol 61:722–727 [View Article][PubMed]
     [Google Scholar]
  12. Liu M., Liu Y., Wang Y., Luo X., Dai J., Fang C. 2011; Lysobacter xinjiangensis sp. nov., a moderately thermotolerant and alkalitolerant bacterium isolated from a gamma-irradiated sand soil sample. Int J Syst Evol Microbiol 61:433–437 [View Article][PubMed]
     [Google Scholar]
  13. Luo G., Shi Z., Wang G. 2012; Lysobacter arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from iron-mined soil. Int J Syst Evol Microbiol 62:1659–1665 [View Article][PubMed]
     [Google Scholar]
  14. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
     [Google Scholar]
  15. Nawrocki E. P., Eddy S. R. 2007; Query-dependent banding (QDB) for faster RNA similarity searches. PLOS Comput Biol 3:e56 [View Article][PubMed]
     [Google Scholar]
  16. Ngo H. T., Won K., Du J., Son H. M., Park Y., Kook M., Kim K. Y., Jin F. X., Yi T. H. 2015; Lysobacter terrae sp. nov. isolated from Aglaia odorata rhizosphere soil. Int J Syst Evol Microbiol 65:587–592 [View Article][PubMed]
     [Google Scholar]
  17. Park J. H., Kim R., Aslam Z., Jeon C. O., Chung Y. R. 2008; Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter . Int J Syst Evol Microbiol 58:387–392 [View Article][PubMed]
     [Google Scholar]
  18. Romanenko L. A., Uchino M., Tanaka N., Frolova G. M., Mikhailov V. V. 2008; Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge. Int J Syst Evol Microbiol 58:370–374 [View Article][PubMed]
     [Google Scholar]
  19. Saddler G. S., Bradbury J. F. 2005; Family I. Xanthomonadaceae fam. nov. In Bergey's Manual of Systematic Bacteriology 2nd edn, vol. 2, pp. 63 (The Proteobacteria), part B (The Gammaproteobacteria). Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [View Article]
     [Google Scholar]
  20. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  21. Singh H., Du J., Won K.-H., Yang J.-E., Akter S., Kim K.-Y., Yi T.-H. 2015; Lysobacter novalis sp. nov., isolated from fallow farmland soil. Int J Syst Evol Microbiol 65:3131–3136 [View Article][PubMed]
     [Google Scholar]
  22. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P. Washington, D.C: American Society for Microbiology;
     [Google Scholar]
  23. Srinivasan S., Kim M. K., Sathiyaraj G., Kim H. B., Kim Y. J., Yang D. C. 2010; Lysobacter soli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 60:1543–1547 [View Article][PubMed]
     [Google Scholar]
  24. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J., other authors. 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Syst Evol Microbiol 52:1043–1047[PubMed]
     [Google Scholar]
  25. Stamatakis A., Ott M., Ludwig T. 2005; RAxML-OMP: An efficient program for phylogenetic inference on SMPs. In Proceedings of 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science 3506 pp 288–302 Springer Verlag
    [Google Scholar]
  26. Ten L. N., Jung H. M., Im W. T., Yoo S. A., Oh H. M., Lee S. T. 2009; Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 59:958–963 [View Article][PubMed]
     [Google Scholar]
  27. Wang Y., Dai J., Zhang L., Luo X., Li Y., Chen G., Tang Y., Meng Y., Fang C. 2009; Lysobacter ximonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 59:786–789 [View Article][PubMed]
     [Google Scholar]
  28. Wei D. Q., Yu T. T., Yao J. C., Zhou E. M., Song Z. Q., Yin Y. R., Ming H., Tang S. K., Li W. J. 2012; Lysobacter thermophilus sp. nov., isolated from a geothermal soil sample in Tengchong, south-west China. Antonie van Leeuwenhoek 102:643–651 [View Article][PubMed]
     [Google Scholar]
  29. Weon H. Y., Kim B. Y., Baek Y. K., Yoo S. H., Kwon S. W., Stackebrandt E., Go S. J. 2006; Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. Int J Syst Evol Microbiol 56:947–951 [View Article][PubMed]
     [Google Scholar]
  30. Yassin A. F., Chen W. M., Hupfer H., Siering C., Kroppenstedt R. M., Arun A. B., Lai W. A., Shen F. T., Rekha P. D., Young C. C. 2007; Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57:1131–1136 [View Article][PubMed]
     [Google Scholar]
  31. Ye X. M., Chu C. W., Shi C., Zhu J. C., He Q., He J. 2015; Lysobacter caeni sp. nov., isolated from the sludge of a pesticide manufacturing factory. Int J Syst Evol Microbiol 65:845–850 [View Article][PubMed]
     [Google Scholar]
  32. Zhang L., Bai J., Wang Y., Wu G. L., Dai J., Fang C. X. 2011; Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 61:2259–2265 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000884
Loading
Lysobacter aestuarii sp. nov., isolated from estuary sediment
Int J Syst Evol Microbiol 66, 1346 (2016); https://doi.org/10.1099/ijsem.0.000884
/content/journal/ijsem/10.1099/ijsem.0.000884
/content/journal/ijsem/10.1099/ijsem.0.000884
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error