Ehrlichia minasensis sp. nov., isolated from the tick Rhipicephalus microplus

Alejandro Cabezas-Cruz; Erich Zweygarth; Marie Vancová; Marzena Broniszewska; Libor Grubhoffer; Lygia Maria Friche Passos; Múcio Flávio Barbosa Ribeiro; Pilar Alberdi; José de la Fuente

doi:10.1099/ijsem.0.000895

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f Ehrlichia minasensis sp. nov., isolated from the tick Rhipicephalus microplus

Authors: Alejandro Cabezas-Cruz^1
,† , Erich Zweygarth^2
,3
,† , Marie Vancová^4
,† , Marzena Broniszewska² , Libor Grubhoffer⁴ , Lygia Maria Friche Passos⁵ , Múcio Flávio Barbosa Ribeiro⁶ , Pilar Alberdi⁷ , José de la Fuente^7
,8
- VIEW AFFILIATIONS
- ¹ 1 Center for Infection and Immunity of Lille (CIIL), INSERM U1019 – CNRS UMR 8204, Université de Lille, Institut Pasteur de Lille, 1 rue Professeur Calmette, 59019 Lille Cedex, France ² 2 Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Leopoldstrasse 5, 80802 Munich, Germany ³ 3 Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa ⁴ 4 University of South Bohemia, Faculty of Science and Biology Centre of the ASCR, Institute of Parasitology, Cˇeské Budeˇjovice, Czech Republic ⁵ 5 Departamento de Medicina Veterinária Preventiva, Escola de Veterinária-UFMG, Belo Horizonte, Minas Gerais, Brazil ⁶ 6 Departamento de Parasitologia, ICB-UFMG, Belo Horizonte, Brazil ⁷ 7 SaBio. Instituto de Investigación en Recursos Cinegéticos IREC, CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain ⁸ 8 Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Correspondence José de la Fuente [email protected]
First Published Online: 01 March 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1426-1430, doi: 10.1099/ijsem.0.000895
Subject: NEW TAXA - Proteobacteria

Cover date: 01/03/2016

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Recently, we obtained a rickettsial isolate (Ehrlichia sp. UFMG-EVT) from the haemolymph of engorged Rhipicephalus microplus tick females. On the basis of maximum-likelihood phylogenetic analysis using 16S rRNA gene, groEL, dsb, gltA and trp36 sequences we showed that Ehrlichia sp. UFMG-EVT belongs to the α-Proteobacteria, family Anaplasmataceae, genus Ehrlichia. Ehrlichia sp. UFMG-EVT is a sister taxon of Ehrlichia canis with 16S rRNA gene, groEL, dsb, gltA and trp36 sequence similarities of 98.3 %, 97.2 %, 94.7 %, 94.3 % and 49.1 %, respectively. Ehrlichia sp. UFMG-EVT has been maintained in the laboratory by continuous passage in the IDE8 tick cell line where the ultrastructure was characterized using electron microscopy and was found to resemble that of E. canis, Ehrlichia muris and Ehrlichia chaffeensis, but not Ehrlichia ruminantium and Ehrlichia ewingii. We propose the name Ehrlichia minasensis sp. nov. for this bacterium to acknowledge the place from where it was initially isolated, Minas Gerais, Brazil. The type strain is strain Ehrlichia sp. UFMG-EVT ( = DSM 100393T = TCB-TBB-0018T).

†
These authors contributed equally to this work.

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