@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000896, author = "Anwar, Nusratgul and Abaydulla, Gulsimay and Zayadan, Bolatkhan and Abdurahman, Mehfuzem and Hamood, Buayshem and Erkin, Raziya and Ismayil, Nurimangul and Rozahon, Manziram and Mamtimin, Hormathan and Rahman, Erkin", title = "Pseudomonas populi sp. nov., an endophytic bacterium isolated from Populus euphratica", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "3", pages = "1419-1425", doi = "https://doi.org/10.1099/ijsem.0.000896", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000896", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-stain negative, aerobic, non-motile, short-rod-shaped bacterium, designated KBL-4-9T, was isolated from the storage liquid in the stems of Populus euphratica trees in Xinjiang, PR China. Strain KBL-4-9T grew at 4–45 °C (optimum 37 °C), 1–3 % (w/v) NaCl (optimum 1 %, w/v) and pH 5.5–9.5 (optimum pH 7.5). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KBL-4-9T belonged to the genus Pseudomonas and showed the highest 16S rRNA gene sequence similarity of 97.36 % to Pseudomonas pelagia CL-AP6T, followed by Pseudomonas xinjiangensis S3-3T (97.16 %), Pseudomonas sabulinigri J64T (97.15 %) and Pseudomonas xiamenensis C10-2T (96.47 %). Analysis of strain KBL-4-9T based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the phylogenetic assignment of the isolates. The DNA G+C content was 61.6 mol% (sd = 2.19). DNA–DNA hybridization with P. pelagia CL-AP6T, P. xinjiangensis S3-3T and P. sabulinigri J64T revealed 49.3 % (sd = 3.04), 41.2 % and 52.5 % (sd = 4.45) relatedness, respectively. The major cellular fatty acids of strain KBL-4-9T were C16 : 0, C12 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C17 : 0 cyclo. The major isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain KBL-4-9T is considered to represents a novel species of the genus Pseudomonas, for which the name Pseudomonas populi sp. nov. is proposed. The type strain is KBL-4-9T ( = JCM 19138T = CCTCC AB 2013069T = NRRL B-59988T).", }