1887

Abstract

Strain BD3526, isolated from raw yak milk collected in Tibet, China, was studied to determine its taxonomic status. The strain was Gram-reaction positive, motile, catalase-positive, aerobic or facultatively anaerobic. The DNA G+C content of the strain was 47.5 mol% and its peptidoglycan contained -diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C, anteiso-C and C. Based on 16S rRNA gene sequence analysis, similarities among strain BD3526 and its most closely related species, A9, ‘’ H9, ‘’ Y24 and DSM 22170 were 99.0, 98.5, 97.3 and 96.7 %, respectively. Levels of DNA–DNA relatedness among strain BD3526 and DSM 22170 and ATCC 842 ( = DSM 36), the type species of the genus, were 41.2 and 45.6 %, respectively. genome-to-genome comparison showed that the DNA–DNA hybridization values among strain BD3526 and A9, ‘’ H9 and ‘’ Y24 were lower than 70 %. Based on its molecular and physiological properties, strain BD3526 ( = DSM 28815 = CGMCC 8333) is identified as the type strain of a novel species within the genus , for which the name is proposed.

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2016-03-01
2024-03-29
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References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 64:253–260 [View Article][PubMed]
    [Google Scholar]
  2. Berge O., Guinebretière M. H., Achouak W., Normand P., Heulin T. 2002; Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Microbiol 52:607–616 [View Article][PubMed]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  4. Elo S., Suominen I., Kämpfer P., Juhanoja J., Salkinoja-Salonen M., Haahtela K. 2001; Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland. Int J Syst Evol Microbiol 51:535–545 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Gouzou L., Burtin G., Philippy R., Bartoli F., Heulin T. 1993; Effect of inoculation with Bacillus polymyxa on soil aggregation in the wheat rhizosphere: preliminary examination. Geoderma 56:479–491 [View Article]
    [Google Scholar]
  7. Haggag W. M. 2007; Colonization of exopolysaccharide-producing Paenibacillus polymyxa on peanut roots for enhancing resistance against crown rot disease. Afr J Biotechnol 6:1568–1577
    [Google Scholar]
  8. Heulin T., Berge O., Mavingui P., Gouzou L., Hebbar K. P., Balandreau J. 1994; Bacillus polymyxa and Rahnella aquatilis, the dominant N2-fixing bacteria associated with wheat rhizosphere in French soils. Eur J Soil Biol 30:35–42
    [Google Scholar]
  9. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  10. Jiang B., Zhao X., Liu J., Fu L., Yang C., Hu X. 2015; Paenibacillus shenyangensis sp. nov., a bioflocculant-producing species isolated from soil under a peach tree. Int J Syst Evol Microbiol 65:220–224 [View Article][PubMed]
    [Google Scholar]
  11. Kanzawa Y., Harada A., Takeuchi A., Yokota A., Harada T. 1995; Bacillus curdlanolyticus sp. nov. and sp. nov., which hydrolyze resistant curdlan. Int J Syst Evol Microbiol 45:515–521[PubMed]
    [Google Scholar]
  12. Liu Y., Liu L., Qiu F., Schumann P., Shi Y., Zou Y., Zhang X., Song W. 2010; Paenibacillus hunanensis sp. nov., isolated from rice seeds. Int J Syst Evol Microbiol 60:1266–1270 [View Article][PubMed]
    [Google Scholar]
  13. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121 [View Article][PubMed]
    [Google Scholar]
  14. Ma Y., Xia Z., Liu X., Chen S. 2007; Paenibacillus sabinae sp. nov., a nitrogen-fixing species isolated from the rhizosphere soils of shrubs. Int J Syst Evol Microbiol 57:6–11 [View Article][PubMed]
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  17. Mavingui P., Heulin T. 1994; In vitro chitinase and antifungal activity of a soil, rhizosphere and rhizoplane population of Bacillus polymyxa . Soil Biol Biochem 26:801–803 [View Article]
    [Google Scholar]
  18. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14:60 [View Article][PubMed]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  20. Montes M. J., Mercadé E., Bozal N., Guinea J. 2004; Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int J Syst Evol Microbiol 54:1521–1526 [View Article][PubMed]
    [Google Scholar]
  21. Nakamura L. K. 1987; Bacillus alginolyticus sp. nov. and Bacillus chondroitinus sp. nov., two alginate-degrading species. Int J Syst Bacteriol 37:284–286 [View Article]
    [Google Scholar]
  22. Pearson W. R. 1990; Rapid and sensitive sequence comparison with fastp fasta . Methods Enzymol 183:63–98 [View Article][PubMed]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  24. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
    [Google Scholar]
  25. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156 [View Article]
    [Google Scholar]
  26. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  27. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [View Article][PubMed]
    [Google Scholar]
  28. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  29. Slepecky R. A., Hemphill H. E. 2006; The genus Bacillus-nonmedical. In The Prokaryotes pp 1663–1696Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [View Article]
    [Google Scholar]
  30. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0.. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  31. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  33. Zhu J., Wang W., Li S. H., Song S. Q., Xie Y. Q., Tang Q. Y., Osman G., Shi Y. H., Zhang Z. D., Li W. J. 2015; Paenibacillus wulumuqiensis sp. nov. and Paenibacillus dauci sp. nov., two novel species of the genus Paenibacillus . Arch Microbiol 197:489–495 [View Article][PubMed]
    [Google Scholar]
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