1887

Abstract

A Gram-stain-positive actinobacterial strain, designated F23, was isolated from marine sediment collected from the western Pacific. Strain F23 showed less than 94.5 % 16S rRNA gene sequence similarity with type strains of species with validly published names. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that the novel isolate formed a distinct monophyletic clade within the family and clustered distantly with the genera and . Cells of strain F23 were non-motile, rod-shaped and aerobic to microaerophilic. Optimal growth occurred at 35–37 °C, at pH 8.0–9.0 and in the presence of 1 % NaCl (w/v). The isolate contained -diaminopimelic acid as the characteristic cell-wall diamino acid, MK-8(H) and MK-7(H) as the predominant menaquinones and anteiso-C and anteiso-C as the major fatty acids. The DNA G+C content of strain F23 was 69.0 mol%. On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics and 16S rRNA gene signature nucleotide patterns, strain F23 represents a novel species in a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is F23 ( = JCM 19771 = CGMCC 1.12785 = MCCC 1A09945).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000909
2016-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/3/1494.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000909&mimeType=html&fmt=ahah

References

  1. Breed R. S. 1953; The families developed from Bacteriaceae Cohn with a description on the family Brevibacteriaceae Breed, 1953. VI Congresso Internazionale Microbiologia Roma 1:10–15 [View Article]
    [Google Scholar]
  2. Cerny G. 1978; Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Appl Microbiol Biotechnol 5:113–122 [View Article]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
    [Google Scholar]
  4. Cui Y., Kang M. S., Woo S. G., Jin L., Kim K. K., Park J., Lee M., Lee S. T. 2013; Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture. Int J Syst Evol Microbiol 63:152–157 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 20:406–416 [View Article]
    [Google Scholar]
  8. Kaiser P., Geyer R., Surmann P., Fuhrmann H. 2012; LC-MS method for screening unknown microbial carotenoids and isoprenoid quinones. J Microbiol Methods 88:28–34 [View Article][PubMed]
    [Google Scholar]
  9. Kates M. 1986; General analytical procedures. Techniques of lipidology 2:112–185
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.other authors 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Kim J., Srinivasan S., You T., Bang J. J., Park S., Lee S. S. 2013; Brevibacterium ammoniilyticum sp. nov. an ammonia-degrading bacterium isolated from sludge of a wastewater treatment plant.. Int J Syst Evol Microbiol 63:1111–1118 [View Article][PubMed]
    [Google Scholar]
  12. Kumar A., Ince, Katı A., Chakraborty R. 2013; Brevibacterium siliguriense sp. nov., a facultatively oligotrophic bacterium isolated from river water. Int J Syst Evol Microbiol 63:511–515 [View Article][PubMed]
    [Google Scholar]
  13. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic acid techniques in bacterial systematics pp 115–175Edited by E. Stackebrandt, Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  14. Lee S. D. 2013; Spelaeicoccus albus gen. nov., sp. nov., an actinobacterium isolated from a natural cave. Int J Syst Evol Microbiol 63:3958–3963 [View Article][PubMed]
    [Google Scholar]
  15. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  18. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system. Actinobacteria classis. nov. Int J Syst Bacteriol 47:479–491 [View Article]
    [Google Scholar]
  19. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0.. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  21. Trujillo M. E., Goodfellow M. 2012; Genus I. Brevibacterium . In Bergey's Manual of Systematic Bacteriology pp 685–700Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B. New York: Springer;
    [Google Scholar]
  22. Xu L. H., Li W. J., Liu Z. H., Jiang C. L.editors 2007; Physiological and biochemical characteristics. In Actinomycete Systematic—Principle, Methods and Practice pp 40–53 Beijing: Science Press;
    [Google Scholar]
  23. Yin X., Yang Y., Wang S., Zhang G. 2015; Virgibacillus oceani sp. nov. isolated from ocean sediment. Int J Syst Evol Microbiol 65:159–164 [View Article][PubMed]
    [Google Scholar]
  24. Zhi X. Y., Li W. J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000909
Loading
/content/journal/ijsem/10.1099/ijsem.0.000909
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error