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f sp. nov., isolated from a patient with systemic protothecosis

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  • Authors: Michiaki Masuda1​ ,  Noriyuki Hirose1​,2​ ,  Tomohiro Ishikawa1​ ,  Yoshiya Ikawa3​ , Kazuko Nishimura1​,4​
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    • 1​ 1​Department of Microbiology, Dokkyo Medical University School of Medicine, Tochigi 321-0293, Japan 2​ 2​BD Japan, Co., Ltd, Tokyo 107-0052, Japan 3​ 3​Laboratory of Biofunctional Chemistry, Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan 4​ 4​First Laboratories, Co. Ltd, Kanagawa 211-0013, Japan
  • Michiaki Masuda [email protected]
  • First Published Online: 01 March 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1510-1520, doi: 10.1099/ijsem.0.000911
  • Subject: NEW TAXA - Eukaryotic micro-organisms
  • Cover date:
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  • Species of the genus are achlorophyllous algae and ubiquitous in nature, and so far, six species have been listed in this genus: , , , , and . A strain of the genus , IFM 53848, was isolated in Japan from a patient with systemic protothecosis and had been designated . Our previous study, by using PCR analysis, revealed that its SSU rRNA gene (rDNA) was distinctively larger than that of and other species of the genus . In this study, molecular analysis showed that the exceptionally large SSU rDNA of IFM 53848 contains four group I introns. The morphology of IFM 53848 was indistinguishable from those of or , and phylogenetic analyses, based on the sequences of the SSU rDNA exons and the D1/D2 region of the large subunit rDNA, indicated that IFM 53848 was closely related to . On the other hand, unlike , IFM 53848 failed to assimilate fructose or lysine and grew well at higher temperatures of up to 42 °C. In addition, the nucleotide sequence of the ribosomal internal transcribed spacer and the matrix assisted laser desorption ionization time-of-flight mass spectrometry profile of IFM 53848 were clearly distinct from those of . The results strongly suggest that IFM 53848 represents a novel species, and so the seventh member of the genus , which we have named sp. nov. The unique characteristics of the strain may provide useful insights into the systematic taxonomy of the genus .

  • The GenBank/EMBL/DDBJ accession numbers for nucleotide sequences of the SSU rDNA, ITS and D1/D2 region of the LSU rDNA of strain IFM 53848 are KP898383, KP898384 and KP898385, respectively. The accession numbers for the sequences of the corresponding regions of strain IFM 54820 are KP898386, KP898387 and KP898388, respectively. The accession number for the ITS sequence of JCM 15793 is KP898389.

  • One supplementary figure is available with the online Supplementary Material.

  • Abbreviations:
    ITS internal transcribed spacer
    MALDI-TOF matrix assisted laser desorption ionization time-of-flight mass spectrometry

© 2016 IUMS | Published by the Microbiology Society

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