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f sp. nov., isolated from faeces of black lemurs ()

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  • Forty-three strains of bifidobacteria were isolated from the faeces of two adult black lemurs, . Thirty-four were identified as , recently described in . The nine remaining isolates were Gram-positive-staining, non-spore-forming, fructose-6-phosphate phosphoketolase-positive, microaerophilic, irregular rod-shaped bacteria that often presented Y- or V-shaped cells. Typing techniques revealed that these isolates were nearly identical, and strain LMM_E3 was chosen as a representative and characterized further. Phylogenetic analysis based on 16S rRNA gene sequences clustered this isolate inside the genus and showed the highest levels of sequence similarity with DSM 28807 (99.3 %), with LMG 21816 and subsp. ATCC 15697 (96.4 and 96.3 %, respectively) as the next most similar strains. The gene sequence of strain LMM_E3 showed the highest similarity to that of DSM 23968 (93.3 %), and 91.0 % similarity to that of the type strain of . DNA–DNA reassociation with the closest neighbour DSM 28807 was found to be 65.4 %. The DNA G+C content was 62.3 mol%. Strain LMM_E3 showed a peptidoglycan structure that has not been detected in bifidobacteria so far: A3α -Lys–-Ser–-Thr–-Ala. Based on the phylogenetic, genotypic and phenotypic data, strain LMM_E3 represents a novel species within the genus , for which the name sp. nov. is proposed; the type strain is LMM_E3 ( = DSM 100216 = JCM 30801).

  • †These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and partial , , , , and gene sequences of strain LMM_E3 are KP979748 and KP979742–KP979747, respectively.

  • Four supplementary figures are available with the online Supplementary Material.

  • Abbreviation:
    F6PPK fructose-6-phosphate phosphoketolase

© 2016 IUMS | Published by the Microbiology Society

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