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f Actinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil

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  • Authors: Kannika Duangmal1​,2​ ,  Paweena Suksaard1​ ,  Wasu Pathom-aree3​ ,  Ratchanee Mingma4​ ,  Atsuko Matsumoto4​ , Yoko Takahashi4​
    • VIEW AFFILIATIONS
    • 1​ 1​Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand 2​ 2​Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok 10900, Thailand 3​ 3​Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand 4​ 4​Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
  • Correspondence Kannika Duangmal [email protected]
  • First Published Online: 01 April 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1660-1665, doi: 10.1099/ijsem.0.000926
  • Subject: NEW TAXA - Actinobacteria
  • Cover date:
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Actinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil, Page 1 of 1

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  • The taxonomic position of the halophilic actinobacterial strain, HS05-03, isolated from solar saltern soil, was determined using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence of the strain showed that it formed a distinct evolutionary lineage in the genus Actinopolyspora. The organism was most closely related to the type strains of the species Actinopolyspora xinjiangensis (98.0 % similarity), Actinopolyspora righensis (97.9 % similarity), Actinopolyspora lacussalsi (97.9 % similarity) and Actinopolyspora erythraea (97.8 % similarity). The whole-organism hydrolysates contained meso-diaminopimelic acid, arabinose, galactose and ribose. The predominant menaquinones were found to be MK-9(H4) and MK-10(H4). The acyl type of the peptidoglycan was N-acetyl. The diagnostic phospholipid detected was phosphatidylcholine. The predominant cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 69.9 mol%. DNA–DNA hybridization values between strain HS05-03 and the type strains of the most closely related species were below the 70 % threshold. On the basis of the phenotypic and genotypic data, it is proposed that strain HS05-03 represents a novel species of the genus Actinopolyspora, with the name Actinopolyspora salinaria sp. nov. The type strain is HS05-03 ( = BCC 51286 = NBRC 109078).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HS05-03 is AB894336.

  • One supplementary figure and one supplementary table are available with the online Supplementary Material.

  • Abbreviations:
    PE phosphatidylethamolamine
    PG phosphatidylglycerol

© 2016 IUMS | Published by the Microbiology Society

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