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- Volume 66, Issue 4
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f Actinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil
- Authors: Kannika Duangmal1,2 , Paweena Suksaard1 , Wasu Pathom-aree3 , Ratchanee Mingma4 , Atsuko Matsumoto4 , Yoko Takahashi4
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1 1Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand 2 2Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok 10900, Thailand 3 3Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand 4 4Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
- Correspondence Kannika Duangmal [email protected]
- First Published Online: 01 April 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1660-1665, doi: 10.1099/ijsem.0.000926
- Subject: NEW TAXA - Actinobacteria
- Cover date:




Actinopolyspora salinaria sp. nov., a halophilic actinomycete isolated from solar saltern soil, Page 1 of 1
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The taxonomic position of the halophilic actinobacterial strain, HS05-03T, isolated from solar saltern soil, was determined using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence of the strain showed that it formed a distinct evolutionary lineage in the genus Actinopolyspora. The organism was most closely related to the type strains of the species Actinopolyspora xinjiangensis (98.0 % similarity), Actinopolyspora righensis (97.9 % similarity), Actinopolyspora lacussalsi (97.9 % similarity) and Actinopolyspora erythraea (97.8 % similarity). The whole-organism hydrolysates contained meso-diaminopimelic acid, arabinose, galactose and ribose. The predominant menaquinones were found to be MK-9(H4) and MK-10(H4). The acyl type of the peptidoglycan was N-acetyl. The diagnostic phospholipid detected was phosphatidylcholine. The predominant cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 69.9 mol%. DNA–DNA hybridization values between strain HS05-03T and the type strains of the most closely related species were below the 70 % threshold. On the basis of the phenotypic and genotypic data, it is proposed that strain HS05-03T represents a novel species of the genus Actinopolyspora, with the name Actinopolyspora salinaria sp. nov. The type strain is HS05-03T ( = BCC 51286T = NBRC 109078T).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HS05-03T is AB894336.
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One supplementary figure and one supplementary table are available with the online Supplementary Material.
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Abbreviations: PE phosphatidylethamolamine PG phosphatidylglycerol
© 2016 IUMS | Published by the Microbiology Society
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