Cohnella saccharovorans sp. nov., isolated from ginseng soil
Choi, Jung-Hye and Seok, Ji-Hye and Jang, Ho-Jin and Cha, Ju-Hee and Cha, Chang-Jun,, 66, 1713-1717 (2016),
doi = https://doi.org/10.1099/ijsem.0.000933,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A novel bacterial strain, CJ22T, was isolated from soil of a ginseng field located in Anseong, Korea. Cells of strain CJ22T were aerobic, Gram-stain-positive, endospore-forming, motile, oxidase- and catalase-positive and rod-shaped. The isolate grew optimally at pH 7 and 30 °C. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CJ22T belonged to the genus Cohnella, displaying highest sequence similarity of 97.3 % with Cohnella panacarvi Gsoil 349T. DNA–DNA relatedness between strain CJ22T and its closest relative was 35.5 % (reciprocal value, 23.8 %). The phenotypic features of strain CJ22T also distinguished it from related species of the genus Cohnella. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major isoprenoid quinone was menaquinone MK-7 and the major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, lysyl-phosphatidylglycerol, two unidentified phospholipids and two unidentified aminophospholipids. The predominant cellular fatty acids of strain CJ22T were anteiso-C15 : 0, iso-C16 : 0 and C16 : 0. The DNA G+C content was 63.1 mol%. Based on data from this polyphasic taxonomic study, strain CJ22T is considered to represent a novel species of the genus Cohnella, for which the name Cohnella saccharovorans sp. nov. is proposed. The type strain is CJ22T ( = KACC 17501T = JCM 19227T).,
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