RT Journal Article SR Electronic(1) A1 Daroonpunt, Rungsima A1 Tanasupawat, Somboon A1 Kudo, Takuji A1 Ohkuma, Moriya A1 Itoh, TakashiYR 2016 T1 Virgibacillus kapii sp. nov., isolated from Thai shrimp paste (Ka-pi) JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 4 SP 1832 OP 1837 DO https://doi.org/10.1099/ijsem.0.000951 PB Microbiology Society, SN 1466-5034, AB A Gram-reaction-positive and rod-shaped bacterium, designated KN3-8-4T, was isolated from shrimp paste collected from a market in Nakhon Sri Thammarat province, Thailand. Strain KN3-8-4T was a strictly aerobic, motile bacterium that produced ellipsoidal endospores at a terminal position. The isolate grew at pH 4.5–10 (optimum pH 7.5), at 11–42 °C (optimum 37 °C) and in the presence of 0–15 % (w/v) NaCl (optimum 1–5 %). On the basis of 16S rRNA gene sequences, strain KN3-8-4T belonged to the genus Virgibacillus and showed highest similarity with Virgibacillus olivae JCM 30551T (97.85 % 16S rRNA gene sequence similarity). The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the major isoprenoid quinone was MK-7. Strain KN3-8-4T contained anteiso-C15 : 0 and anteiso-C17 : 0 as major cellular fatty acids and had phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and one glycolipid as polar lipids. The DNA G+C content was 43.5 mol%. Strain KN3-8-4T showed low DNA–DNA relatedness (20.44 %) with V. olivae JCM 30551T. On the basis of phenotypic and chemotaxonomic data and phylogenetic analyses, strain KN3-8-4T represents a novel species of the genus Virgibacillus for which the name Virgibacillus kapii sp. nov. is proposed. The type strain is KN3-8-4T ( = JCM 30071T = LMG 28282T = TISTR 2279T = PCU 345T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000951