@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000957, author = "Zhu, Daochen and Zhang, Peipei and Li, Pingping and Wu, Jian and Xie, Changxiao and Sun, Jianzhong and Niu, Lili", title = "Description of Leucobacter holotrichiae sp. nov., isolated from the gut of Holotrichia oblita larvae", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "4", pages = "1857-1861", doi = "https://doi.org/10.1099/ijsem.0.000957", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000957", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-stain-positive bacterium, designated T14T, was isolated from the gut of Holotrichia oblita larvae and was subjected to a taxonomic study. The isolate was rod-shaped, aerobic, non-motile, non-spore-forming and yellow-pigmented. Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that the isolate is related to the genus Leucobacter. Its closest neighbours were the type strains ‘Leucobacter kyeonggiensis’ F3-P9 (96.8 % 16S rRNA gene sequence similarity), Leucobacter celer NAL101T (96.2 %) and Leucobacter chironomi DSM 19883T (95.5 %). The DNA G+C content of strain T14T was 69.3 mol%, and DNA–DNA hybridization values with closely related strains were < 32 %. The predominant cellular fatty acids were anteiso-C15 : 0 (49.3 %), iso-C16 : 0 (16.4 %) and anteiso-C17 : 0 (16.8 %). The major polar lipids were aminolipid, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphoglycolipid and unidentified glycolipids. The predominant respiratory quinone was MK-11. Based on these phylogenetic and phenotypic results, strain T14T can be clearly distinguished from all of the recognized species of the genus Leucobacter and is considered to represent a novel species of the genus Leucobacter. The name Leucobacter holotrichiae sp. nov. is proposed, with the type strain T14T ( = DSM 28968T = JCM 30245T).", }