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f Nocardioides zeicaulis sp. nov., an endophyte actinobacterium of maize

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  • Authors: Peter Kämpfer1​ ,  Stefanie P. Glaeser1​ ,  John A. McInroy2​ , Hans-Jürgen Busse3​
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    • 1​ 1​ Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392, Giessen, Germany 2​ 2​ Entomology and Plant Pathology Dept., Auburn University, Alabama, AL 36849, USA 3​ 3​ Abteilung für Klinische Mikrobiologie und Infektionsbiologie, Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, A-1210 Wien, Austria
  • Correspondence Peter Kämpfer [email protected]
  • First Published Online: 01 April 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1869-1874, doi: 10.1099/ijsem.0.000959
  • Subject: NEW TAXA - Actinobacteria
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Nocardioides zeicaulis sp. nov., an endophyte actinobacterium of maize, Page 1 of 1

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  • A Gram-stain-positive, aerobic organism was isolated as an endophyte from the stem tissue of healthy maize (Zea mays) and investigated in detail for its taxonomic position. On the basis of the 16S rRNA gene sequence analysis, strain JM-601 was shown to be most closely related to Nocardioides alpinus (98.3 %), and Nocardioides ganghwensis (98.0 %). The 16S rRNA gene sequence similarity to all other species of the genus Nocardioides was ≤ 98.0 %. The diagnostic diamino acid of the peptidoglycan was ll-diaminopimelic acid. The major quinone of strain JM-601 was menaquinone MK-8(H4). The polar lipid profile revealed the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylcholine and an unidentified phospholipid. The polyamine pattern contained predominantly spermine and moderate amounts of spermidine. In the fatty acid profile, iso-C16 : 0, C17 : 1ω8c and 10-methyl C17 : 0 were present in major amounts. All these data support the allocation of the strain to the genus Nocardioides. The results of physiological and biochemical characterization allow in addition a phenotypic differentiation of strain JM-601 from N. alpinus and N. ganghwensis. Strain JM-601 represents a novel species of the genus Nocardioides, for which we propose the name Nocardioides zeicaulis sp. nov., with JM-601 ( = CCM 8654 = CIP 110980) as the type strain.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JM-601 is KU201963.

  • Two supplementary figures are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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