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f sp. nov., isolated from freshwater sediment

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  • Authors: Long Jin1 ,  So-Ra Ko2 ,  Chi-Yong Ahn2 ,  Hyung-Gwan Lee3 , Hee-Mock Oh2
    • VIEW AFFILIATIONS
    • 1 1College of Biology and the Environment, Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210-037, PR China 2 2Bioenergy and Biochemical Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea 3 3Sustainable Biomass Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea
  • Hyung-Gwan Lee [email protected]
  • First Published Online: 01 May 2016, International Journal of Systematic and Evolutionary Microbiology 66: 1926-1931, doi: 10.1099/ijsem.0.000962
  • Subject: NEW TAXA - Proteobacteria
  • Cover date:
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  • Three bacterial strains, designated DS48-6-5, DS48-6-7 and DS48-6-9, were isolated from a sediment sample taken from Daechung Reservoir (Republic of Korea) at a water depth of 48 m. Cells of the strains were Gram-stain-negative, aerobic, rod-shaped and motile with a single polar flagellum. Comparative 16S rRNA gene sequence studies showed that the three isolates had clear affiliation with and the closest relatives were KCTC 32299, DSM 11587 and KCTC 12591 with 97.2–97.9 % 16S rRNA gene sequence similarities; the 16S rRNA gene sequence similarities between the three strains were 99.5–100 %. The only isoprenoid quinone of the three strains was ubiquinone-8, and the major fatty acids were summed feature 3 (Cω6 and/or Cω7) and C. The G+C content of the genomic DNA of strains DS48-6-5, DS48-6-7 and DS48-6-9 was 66.7, 67.0 and 66.8 mol%, respectively. DNA–DNA hybridization values of the novel strains with KCTC 32299, DSM 11587 and KCTC 12591 were 19.3–48.5 %. Based on the evidence from this taxonomic study using a polyphasic approach, it is proposed that strains, DS48-6-5, DS48-6-7 and DS48-6-9, represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS48-6-5 ( = KCTC 42645 = NBRC 111169).

  • The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains DS48-6-5, DS48-6-7 and DS48-6-9 are KM670026 KM670027 and KM670028, respectively.

  • A supplementary figure and a supplementary table are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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