%0 Journal Article %A Huang, Jian-Rong %A Ming, Hong %A Li, Shuai %A Meng, Xiao-Lin %A Zhang, Jian-Xin %A Khieu, Thi-Nhan %A Tang, Zhong %A Li, Wen-Jun %A Nie, Guo-Xing %T Agromyces insulae sp. nov., an actinobacterium isolated from a soil sample %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 5 %P 2002-2007 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.000978 %I Microbiology Society, %X A novel Gram-reaction-positive, non-motile, aerobic bacterium, designated CFH S0483T, was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. 16S rRNA gene sequence analysis showed that the strain is a member of the genus Agromyces and has highest 16S rRNA gene sequence similarities with Agromyces humatus DSM 16389T (97.3 %) and Agromyces ramosus DSM 43045T (97.1 %), and similarities  < 97.0 % with type strains of other species of the genus Agromyces. Strain CFH S0483T was able to grow at 10–37 °C, at pH 7.0–9.0 and tolerated NaCl up to 2.0 % (w/v). The whole-cell sugars were mannose, galactose, glucose and ribose. The isolate contained l-2,4-diaminobutyric acid, d-alanine, d-glutamic acid and glycine in the cell-wall peptidoglycan. Strain CFH S0483T exhibited a menaquinone system with MK-12, and the major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The genomic DNA G+C content of strain CFH S0483T was 71.6 mol%. Based on the phylogenetic and phenotypic analysis, and low DNA–DNA hybridization values, strain CFH S0483T could not be classified into any recognized species of the genus Agromyces. Strain CFH S0483T is therefore considered to represent a novel species of the genus Agromyces, for which the name Agromyces insulae sp. nov. is proposed. The type strain is CFH S0483T ( = KCTC 39117T = CCTCC AB 2014301T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000978