@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000983, author = "Xu, Huan and Zhang, Sheng and Cheng, Juan and Asem, Mipeshwaree Devi and Zhang, Meng-Yue and Manikprabhu, Deene and Zhang, Tian-Yuan and Wu, Ying-Ying and Li, Wen-Jun and Zhang, Yi-Xuan", title = "Nocardioides ginkgobilobae sp. nov., an endophytic actinobacterium isolated from the root of the living fossil Ginkgo biloba L.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "5", pages = "2013-2018", doi = "https://doi.org/10.1099/ijsem.0.000983", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000983", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-stain-positive, aerobic and yellow actinobacterial strain, designated SYP-A7303T, was isolated from the root of Ginkgo biloba L. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SYP-A7303T belongs to the genus Nocardioides. The 16S rRNA gene sequence of strain SYP-A7303T showed highest similarity to Nocardioides marinus CL-DD14T ( = JCM 15615T) (98.3 %) and Nocardioides aquiterrae GW-9T ( = JCM 11813T) (97.1 %), and less than 96.9 % to the type strains of other species of the genus Nocardioides. Strain SYP-A7303T grew optimally at 28 °C, pH 7.0 and in the absence of NaCl. It contained ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, with mannose, ribose, rhamnose, glucose and galactose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown lipid. The menaquinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. The DNA G+C content was 72 mol%. Mean DNA–DNA relatedness values between strain SYP-A7303T and the closely related strains N. marinus JCM 15615T and N. aquiterrae JCM 11813T were 62.5 ± 2.4 and 56.5 ± 3.5 %, respectively. Based on the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain SYP-A7303T represents a novel species of the genus Nocardioides, for which the name Nocardioides ginkgobilobae sp. nov. is proposed. The type strain is SYP-A7303T ( = DSM 100492T = KCTC 39594T).", }