RT Journal Article SR Electronic(1) A1 Xu, Huan A1 Zhang, Sheng A1 Cheng, Juan A1 Asem, Mipeshwaree Devi A1 Zhang, Meng-Yue A1 Manikprabhu, Deene A1 Zhang, Tian-Yuan A1 Wu, Ying-Ying A1 Li, Wen-Jun A1 Zhang, Yi-XuanYR 2016 T1 Nocardioides ginkgobilobae sp. nov., an endophytic actinobacterium isolated from the root of the living fossil Ginkgo biloba L. JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 5 SP 2013 OP 2018 DO https://doi.org/10.1099/ijsem.0.000983 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, aerobic and yellow actinobacterial strain, designated SYP-A7303T, was isolated from the root of Ginkgo biloba L. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SYP-A7303T belongs to the genus Nocardioides. The 16S rRNA gene sequence of strain SYP-A7303T showed highest similarity to Nocardioides marinus CL-DD14T ( = JCM 15615T) (98.3 %) and Nocardioides aquiterrae GW-9T ( = JCM 11813T) (97.1 %), and less than 96.9 % to the type strains of other species of the genus Nocardioides. Strain SYP-A7303T grew optimally at 28 °C, pH 7.0 and in the absence of NaCl. It contained ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, with mannose, ribose, rhamnose, glucose and galactose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown lipid. The menaquinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. The DNA G+C content was 72 mol%. Mean DNA–DNA relatedness values between strain SYP-A7303T and the closely related strains N. marinus JCM 15615T and N. aquiterrae JCM 11813T were 62.5 ± 2.4 and 56.5 ± 3.5 %, respectively. Based on the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain SYP-A7303T represents a novel species of the genus Nocardioides, for which the name Nocardioides ginkgobilobae sp. nov. is proposed. The type strain is SYP-A7303T ( = DSM 100492T = KCTC 39594T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000983