1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 5

sp. nov., a haloalkaliphilic bacterium from a coastal-marine wetland Free

Abstract

A novel Gram-stain-negative, straight rod-shaped, non-pigmented, slightly halophilic and alkaliphilic bacterium, designated strain GBPy7, was isolated from a sample of the coastal-marine wetland Gomishan in Iran. Cells of strain GBPy7 were motile. Growth occurred on media with 1–15 % (w/v) NaCl (optimum 3 %), at pH 7–10 (optimum pH 8.5) and at 4–45 °C (optimum 37 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that strain GBPy7 belonged to the family . Its closest relatives were AIS (98.1 % 16S rRNA gene sequence similarity) and other species (95.9–94.2 %), together with CL-SP19 (94.3 %) and F23 (94.3 %). The major cellular fatty acids of the isolate were iso-C, iso-C, iso-Cω9 and Cω7 and its polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown aminophospholipid. Cells of strain GBPy7 contained ubiquinone Q-8. The G+C content of the genomic DNA of this strain was 51.6 mol%. The level of DNA–DNA relatedness between strain GBPy7 and IBRC-M 10414 was 21 %. The physiological, biochemical, genotypic and phylogenetic differences between strain GBPy7 and other previously described taxa indicate that the strain represents a novel species of the genus within the family , for which the name sp. nov. is proposed. The type strain is GBPy7 ( = IBRC-M 10763 = CECT 8339).

  • Published Online:
© 2016 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000996
2016-05-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/5/2099.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000996&mimeType=html&fmt=ahah

References

  1. Atlas R. M. 2005 Media for Environmental Microbiology, 2nd edn. Boca Raton: Taylor and Francis Group;
     [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
     [Google Scholar]
  3. Chiu H.-H., Rogozin D. Y., Huang S.-P., Degermendzhy A. G., Shieh W. Y., Tang S.-L. 2014; Aliidiomarina shirensis sp. nov., a halophilic bacterium isolated from Shira Lake in Khakasia, southern Siberia, and a proposal to transfer Idiomarina maris to the genus Aliidiomarina . Int J Syst Evol Microbiol 64:1334–1339 [View Article][PubMed]
     [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
     [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  7. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
     [Google Scholar]
  8. Harrigan W. F., McCance M. E. 1976 Laboratory Methods in Food and Dairy Microbiology London: Academic Press;
     [Google Scholar]
  9. Huang S. P., Chang H. Y., Chen J. S., Jean W. D., Shieh W. Y. 2012; Aliidiomarina taiwanensis gen. nov., sp. nov., isolated from shallow coastal water. Int J Syst Evol Microbiol 62:155–161 [View Article][PubMed]
     [Google Scholar]
  10. Huss V. A., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
     [Google Scholar]
  11. Ivanova E. P., Romanenko L. A., Chun J., Matte M. H., Matte G. R., Mikhailov V. V., Svetashev V. I., Huq A., Maugel T., Colwell R. R. 2000; Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50:901–907 [View Article][PubMed]
     [Google Scholar]
  12. Ivanova E. P., Flavier S., Christen R. 2004; Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54:1773–1788 [View Article][PubMed]
     [Google Scholar]
  13. Jean W. D., Shieh W. Y., Chiu H. H. 2006; Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . Int J Syst Evol Microbiol 56:899–905 [View Article][PubMed]
     [Google Scholar]
  14. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
     [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  16. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  17. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
     [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
     [Google Scholar]
  19. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. 2002; A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [View Article][PubMed]
     [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high  performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  21. Monciardini P., Cavaletti L., Schumann P., Rohde M., Donadio S. 2003; Conexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria . Int J Syst Evol Microbiol 53:569–576 [View Article][PubMed]
     [Google Scholar]
  22. Murray R.G.E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp 21–41Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  23. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. 1984; Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34:287–292 [View Article]
     [Google Scholar]
  24. Reasoner D. J., Geldreich E. E. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7[PubMed]
     [Google Scholar]
  25. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  26. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  27. Srinivas T.N.R., Nupur, Anil Kumar P. 2012; Aliidiomarina haloalkalitolerans sp. nov., a marine bacterium isolated from coastal surface seawater. Antonie van Leeuwenhoek 101:761–768 [View Article][PubMed]
     [Google Scholar]
  28. Stamatakis A. 2006; RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690 [View Article][PubMed]
     [Google Scholar]
  29. Taborda M., Antunes A., Tiago I., Veríssimo A., Nobre M. F., da Costa M. S. 2009; Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina . Syst Appl Microbiol 32:371–378 [View Article][PubMed]
     [Google Scholar]
  30. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
     [Google Scholar]
  31. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
     [Google Scholar]
  32. Wang G., Wu H., Zhang X., Zhang H., Yang X., Tian X., Li J., Xiang W., Li X. 2013; Aliidiomarina sanyensis sp. nov., a hexabromocyclododecane assimilating bacterium from the pool of Spirulina platensis cultivation, Sanya, China. Antonie van Leeuwenhoek 104:309–314 [View Article][PubMed]
     [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  34. Zhang Y.-J., Zhang X.-Y., Zhao H.-L., Zhou M.-Y., Li H.-J., Gao Z.-M., Chen X.-L., Dang H.-Y., Zhang Y.-Z. 2012; Idiomarina maris sp. nov., a marine bacterium isolated from sediment. Int J Syst Evol Microbiol 62:370–375 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000996
Loading
Aliidiomarina iranensis sp. nov., a haloalkaliphilic bacterium from a coastal-marine wetland
Int J Syst Evol Microbiol 66, 2099 (2016); https://doi.org/10.1099/ijsem.0.000996
/content/journal/ijsem/10.1099/ijsem.0.000996
/content/journal/ijsem/10.1099/ijsem.0.000996
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error