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Volume 66, Issue 6

sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment Free

Abstract

A halotolerant, Gram-stain-negative, rod-shaped and light-pink-pigmented bacterial strain, PB3, was isolated from a pond sediment near a hexachlorocyclohexane-producing factory, located at Chinhat, Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PB3 formed a distinct phyletic clade along with the members of the genus The 16S rRNA gene sequence similarity with other members of the genus ranged from 94.5 to 98.9 %. The cells were motile, aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C, iso-C 3-OH, iso-C3-OH, Cω5, summed feature 3 (Cω6/Cω7) and summed feature 4 (iso-CI/ anteiso-C B). The polar lipid profile of strain PB3 showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids and other unknown polar lipids. DNA–DNA hybridization based homology of strain PB3 with respect to its most closely related species, LP51, was 44.7 %. The DNA G+C content was 53.5 mol%. On the basis of these data, it is proposed that the isolate belongs to the genus and represents a novel species, for which the name is proposed. The type strain is PB3 (=DSM 100162=KCTC 42942).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): gram-negative , hexachlorocyclohexane , mucosus , pond sediment and Pontibacter
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2016-06-10
2024-04-23
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Pontibacter mucosus sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment
Int J Syst Evol Microbiol 66, 2234 (2016); https://doi.org/10.1099/ijsem.0.001013
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