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Volume 66, Issue 6

sp. nov., an actinobacterium isolated from a coastal wetland of the Yellow River Delta Free

Abstract

A Gram-stain-positive, heterotrophic, non-spore-forming, rod-shaped strain, designated OAct353, belonging to the genus was isolated from a soil sample collected from a coastal wetland of the Yellow River delta, PR China. The strain was identified using a polyphasic taxonomic approach. The strain grew in the presence of 0–10 % (w/v) NaCl (optimum 2–3 %), at pH 5.0–8.0 (optimum pH 7.0) and 12–36 °C (optimum 28 °C). The isolate contained 2,4-diaminobutyric acid, glutamic acid and glycine in its peptidoglycan. The acyl type of the cell-wall muramic acid was -acetyl. The whole-cell sugars of this novel strain were glucose, xylose and rhamnose. The predominant menaquinones were MK-12 (74 %) and MK-11 (21 %). The major phospholipids were phosphatidylglycerol, one unknown phospholipid, three unknown glycolipids and three unknown polar lipids. The major fatty acids were iso-C, anteiso-C and anteiso-C. The DNA G+C content was 69.6 mol %. DNA-DNA relatedness clearly separated strain OAct353 from its closest relatives. On the basis of phenotypic, phylogenetic and chemotaxonomic data, a novel species, sp. nov., is proposed. The type strain is OAct353 (=CGMCC4.7180=DSM 28305=NRRL B-59115).

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  • Accepted:
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Keyword(s): actinobacterium , Agromyces and Novel species
© 2016 IUMS
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2016-06-10
2024-04-19
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Agromyces binzhouensis sp. nov., an actinobacterium isolated from a coastal wetland of the Yellow River Delta
Int J Syst Evol Microbiol 66, 2278 (2016); https://doi.org/10.1099/ijsem.0.001022
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