@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001025, author = "Ee, Robson and Madhaiyan, Munusamy and Ji, Lianghui and Lim, Yan-Lue and Nor, Nuruddin Muhammad and Tee, Kok-Keng and Chen, Jian-Woon and Yin, Wai-Fong", title = "Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "6", pages = "2297-2304", doi = "https://doi.org/10.1099/ijsem.0.001025", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001025", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Multilocus sequence analyses", keywords = "Dumping ground", keywords = "RpoB gene", keywords = "Chitinase operon", keywords = "Polyphasic identification", keywords = "Complete genome sequence", keywords = "Quorum sensing", abstract = "Phylogenetic and taxonomic characterization was performed for bacterium RB-25T, which was isolated from a soil sample collected in a former municipal landfill site in Puchong, Malaysia. Growth occurred at 20–37 °C at pH 5–8 but not in the presence of 9 % (w/v) NaCl or higher. The principal fatty acids were C16:0, C18:1ω7c and summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH). Ubiquinone-8 was the only isoprenoid quinone detected. Polar lipid analysis revealed the presence of phospholipid, phosphoaminolipid, phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. DNA G+C content was 50.9 mol% phylogenetic analysis based on 16S rRNA gene sequence showed that strain RB-25T formed a distinct lineage within the family Enterobacteriaceae of the class Gammaproteobacteria . It exhibited a low level of 16S rRNA gene sequence similarity with its phylogenetic neighbours Pantoea rwandensis LMG 26275T (96.6 %), Rahnella aquatilis CIP 78.65T (96.5 %), Pectobacterium betavasculorum ATCC 43762T (96.4 %), Pantoea rodasii LMG 26273T (96.3 %), Gibbsiella dentisursi NUM 1720T (96.3 %) and Serratia glossinae C1T (96.2 %). Multilocus sequence analyses based on fusA, pyrG, rplB, rpoB and sucA sequences showed a clear distinction of strain RB-25T from the most closely related genera. Isolate RB-25T could also be distinguished from members of these genera by a combination of the DNA G+C content, respiratory quinone system, fatty acid profile, polar lipid composition and other phenotypic features. Strain RB-25T represents a novel species of a new genus, for which the name Chania multitudinisentens gen. nov., sp. nov. is proposed. The type strain is RB-25T (=DSM 28811T=LMG 28304T).", }