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f Bacillus urumqiensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a salt lake

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  • Authors: Shanshan Zhang1​ ,  Zhaojun Li2​ ,  Yanchun Yan1​ ,  Chuanlun Zhang3​ ,  Jun Li2​ , Baisuo Zhao1​
    • VIEW AFFILIATIONS
    • 1​ 1​ Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2​ 2​ Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China 3​ 3​ The School of Ocean and Earth Sciences, Tongji University, Shanghai 200092, China
  • Correspondence Baisuo Zhao [email protected]
  • First Published Online: 10 June 2016, International Journal of Systematic and Evolutionary Microbiology 66: 2305-2312, doi: 10.1099/ijsem.0.001028
  • Subject: New taxa - Firmicutes and related organisms
  • Received:
  • Accepted:
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  • A Gram-stain-positive, rod-shaped, aerobic and moderately haloalkaliphilic bacterium, designated BZ-SZ-XJ18, was isolated from the mixed water and sediment of a saline-alkaline lake located in the Xinjiang Uyghur Autonomous Region of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BZ-SZ-XJ18 was a member of the genus Bacillus . The closest phylogenetic relatives were Bacillus saliphilus 6AG (96.7 % 16S rRNA gene sequence similarity), ‘ Bacillus daqingensis’ X10-1 (96.6 %), Bacillus luteus JC167 (96.5 %), Bacillus daliensis DLS13 (96.2 %), Bacillus chagannorensis CG-15 (95.2 %) and Bacillus polygoni YN-1 (95.0 %). DNA–DNA relatedness between strain BZ-SZ-XJ18 and the reference type strains of the related species of the genus Bacillus was lower than 27 %. The isolate formed yellow pigment and grew in the presence of 0.22–4.32 M Na (equivalent to 1.3–25.3 %, w/v, NaCl) (optimum 1.08 M Na, equivalent to 6.3 %, w/v, NaCl), at pH 6.5–10.0 (optimum pH 8.5–9.5) and at 8–41 ºC (optimum 37 ºC). The major cellular fatty acids were anteiso-C15:0 (43.0 %), C16:0 (18.1 %), iso-C15:0 (11.3 %), anteiso-C17:0 (8.0 %) and iso-C16:0 (7.0 %). The major polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The main respiratory quinone was menaquinone-7 (MK-7), and the peptidoglycan type of the cell wall was A1γ based on meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 42.3 mol% (HPLC) or 41.4 mol% (T m). On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BZ-SZ-XJ18 is proposed to represent a novel species, Bacillus urumqiensis within the genus Bacillus . The type strain is BZ-SZ-XJ18 (=DSM 29145=JCM 30195).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BZ-SZ-XJ18 is KM066107.

  • Two supplementary figures and one supplementary table are available with the online Supplementary Material.

  • Abbreviations:
    NJ neighbour-joining
    ME minimum-evolution
    ML maximum-likelihood
    PL phospholipid
    PE phosphatidylethanolamine
    PG phosphatidylglycerol
    DPG diphosphatidylglycerol
    SQDG sulfoquinovosyldiacylglycerol
    MK-7 menaquinone–7

© 2016 IUMS | Published by the Microbiology Society

  • Subhash Y. , Sasikala C. , Ramana C. . ( 2014;). Bacillus luteus sp. nov., isolated from soil. . Int J Syst Evol Microbiol 64: 1580––1586. [CrossRef] [PubMed]
    [Google Scholar]
  • Aino K. , Hirota K. , Matsuno T. , Morita N. , Nodasaka Y. , Fujiwara T. , Matsuyama H. , Yoshimune K. , Yumoto I. . ( 2008;). Bacillus polygoni sp. nov., a moderately halophilic, non-motile obligate alkaliphile isolated from indigo balls. . Int J Syst Evol Microbiol 58: 120––124. [CrossRef] [PubMed]
    [Google Scholar]
  • Amoozegar M. A. , Didari M. , Bagheri M. , Fazeli S. A. , Schumann P. , Spröer C. , Sánchez-Porro C. , Ventosa A. . ( 2013;). Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 63: 3324––3329. [CrossRef] [PubMed]
    [Google Scholar]
  • Bagheri M. , Didari M. , Amoozegar M. A. , Schumann P. , Sánchez-Porro C. , Mehrshad M. , Ventosa A. . ( 2012;). Bacillus iranensis sp. nov., a moderate halophile from a hypersaline lake. . Int J Syst Evol Microbiol 62: 811––816. [CrossRef] [PubMed]
    [Google Scholar]
  • Burdon K. L. . ( 1946;). Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. . J Bacteriol 52: 665––678.[PubMed]
    [Google Scholar]
  • Carrasco I. J. , Márquez M. C. , Xue Y. , Ma Y. , Cowan D. A. , Jones B. E. , Grant W. D. , Ventosa A. . ( 2007;). Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in inner mongolia, china. . Int J Syst Evol Microbiol 57: 2084––2088. [CrossRef] [PubMed]
    [Google Scholar]
  • Cohn F. . ( 1872;). Untersuchungen über bakterien. . Beitr Biol Pflanz 1: 127––244.
     [Google Scholar]
  • Cowan S. , Steel K. . ( 1993;). Manual for the Identification of Medical Bacteria, , 3rd edn.. Edited and revised by Barrow G. I. , Feltham R. K. A. . London:: Cambridge University Press;.
     [Google Scholar]
  • Doetsch R. N. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. H. . ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology. pp. 21––33. Edited by Gerhardt P. . Washington:: American Society for Microbiology;.
     [Google Scholar]
  • Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: A maximum likelihood approach. . J Mol Evol 17: 368––376. [CrossRef] [PubMed]
    [Google Scholar]
  • Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  • Horner-Devine M. C. , Lage M. , Hughes J. B. , Bohannan B. J. M. . ( 2004;). A taxa–area relationship for bacteria. . Nature 432: 750––753. [CrossRef]
    [Google Scholar]
  • Jukes T. H. , Cantor C. R. . ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism,vol. 3 pp. 21––132. Edited by Munro H. N. . New York:: Academic Press;.[CrossRef]
    [Google Scholar]
  • Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716––772. [CrossRef] [PubMed]
    [Google Scholar]
  • Kosowski K. , Schmidt M. , Pukall R. , Hause G. , Kämpfer P. , Lechner U. . ( 2014;). Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov., isolated from a composting reactor. . Int J Syst Evol Microbiol 64: 88––94. [CrossRef] [PubMed]
    [Google Scholar]
  • Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. , other authors . ( 2007;). CLUSTAL W and CLUSTAL_X version 2. . Bioinformatics. 23: 2947––2948.[CrossRef]
    [Google Scholar]
  • Lee S. Y. , Choi J. I. . ( 2004;). Polyhydroxyalkanoates: biodegradeable polymer. . In Manual of Indusrial Microbiology and Biotechnology, , 2nd edn., pp. 616––624. Edited by Demain A. L. , Davies J. E. , Atlas R. M. , Cohen G. , Hershberger C. L. , Hu W. S. , Sherman D. H. , Willson R. C. , David J. H. . Washington:: American Society of Microbiology;.
     [Google Scholar]
  • Lim J. M. , Jeon C. O. , Kim C. J. . ( 2006;). Bacillus taeanensis sp. nov., a halophilic gram-positive bacterium from a solar saltern in korea. . Int J Syst Evol Microbiol 56: 2903––2908. [CrossRef] [PubMed]
    [Google Scholar]
  • Logan N. A. , Popovitch T. J. , Hoffmaster A. . ( 2007;). Bacillus and related genera. . In Manual of Clinical Microbiology, , 9th edn.,vol. 1 pp. 445––460. Edited by Murray P. R. , Baron E. J. , Jorgensen J. H. , Pfaller M. A. , Yolken R. H. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  • Logan N. A. , Berge O. , Bishop A. H. , Busse H. J. , De Vos P. , Fritze D. , Heyndrickx M. , Kämpfer P. , Rabinovitch L. et al. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59: 2114––2121. [CrossRef] [PubMed]
    [Google Scholar]
  • Logan N. A. , De Vos P. . ( 2009;). Genus Bacillus Cohn 1872. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 3 pp. 21––218. Edited by De Vos P. , Garrity G. , Jones D. , Krieg N. R. , Ludwig W. , Rainey F. A. , Schleifer K.-H. , Whitman W. B. . New York:: Springer-Verlag;.
     [Google Scholar]
  • Lányí B. . ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19: 1––67. [CrossRef]
    [Google Scholar]
  • Mata J. A. , Martínez-Cánovas J. , Quesada E. , Béjar V. . ( 2002;). A detailed phenotypic characterisation of the type strains of halomonas species. . Syst Appl Microbiol 25: 360––375. [CrossRef] [PubMed]
    [Google Scholar]
  • Mehrshad M. , Amoozegar M. A. , Didari M. , Bagheri M. , Fazeli S. A. , Schumann P. , Spröer C. , Sánchez-Porro C. , Ventosa A. . ( 2013;). Bacillus halosaccharovorans sp. nov., a moderately halophilic bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 63: 2776––2781. [CrossRef] [PubMed]
    [Google Scholar]
  • Mesbah M , Premachandran U. , Whitman W. B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39: 159––167.[CrossRef]
    [Google Scholar]
  • Mesbah M. , Whitman W. B. . ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479: 297––306. [CrossRef]
    [Google Scholar]
  • Murray R. G. E. , Doetsch R. N. , Robinow C. F. . ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, , 2nd edn., pp. 21––41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . Washington, DC, USA:: American Society for Microbiology;.
     [Google Scholar]
  • Myers E. W. , Miller W. . ( 1988;). Optimal alignments in linear space. . Comput Appl Biosci 4: 11––17. [CrossRef] [PubMed]
    [Google Scholar]
  • Márquez M. C. , Carrasco I. J. , de la Haba R. R. , Jones B. E. , Grant W. D. , Ventosa A. . ( 2011;). Bacillus locisalis sp. nov., a new haloalkaliphilic species from hypersaline and alkaline lakes of china, kenya and tanzania. . Syst Appl Microbiol 34: 424––428. [CrossRef] [PubMed]
    [Google Scholar]
  • Romano I. , Lama L. , Nicolaus B. , Gambacorta A. , Giordano A. . ( 2005;). Bacillus saliphilus sp. nov., isolated from a mineral pool in campania, italy. . Int J Syst Evol Microbiol 55: 159––163. [CrossRef] [PubMed]
    [Google Scholar]
  • Rzhetsky A. , Nei M. . ( 1992;). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9: 945––967.
     [Google Scholar]
  • Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: A new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406––425.[PubMed]
    [Google Scholar]
  • Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. . MIDI Technical Note No. 101, MIDI. NEWARK:: DE. May (Revised Feb 1997);.
     [Google Scholar]
  • Schumann P. . ( 2011;). Peptidoglycan structure. . Methods Microbiol 38: 101––129. [CrossRef]
    [Google Scholar]
  • Stackebrandt E. , Goebel B. M. . ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44: 846––849.[CrossRef]
    [Google Scholar]
  • Sylvan J. B. , Hoffman C. L. , Momper L. M. , Toner B. M. , Amend J. P. , Edwards K. J. . ( 2015;). Bacillus rigiliprofundi sp. nov., an endospore-forming, mn-oxidizing, moderately halophilic bacterium isolated from deep subseafloor basaltic crust. . Int J Syst Evol Microbiol 65: 1992––1998. [CrossRef] [PubMed]
    [Google Scholar]
  • Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). MEGA6: Molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725––2729. [CrossRef] [PubMed]
    [Google Scholar]
  • Tindall B. J. . ( 1990a;). A comparative study of the lipid composition of halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13: 128––130. [CrossRef]
    [Google Scholar]
  • Tindall B. J. . ( 1990b;). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Letts 66: 199––202. [CrossRef]
    [Google Scholar]
  • Tindall B. J. , Smibert R. A. , Sikorski J. , Krieg N. R. . ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn.,vol. 1 pp. 330––393. Edited by Reddy C. A. , Beveridge T. J. , Breznak J. A. , Marzluf G. , Schmidt T. M. , Snyder L. R. . Washington DC, USA:: ASM Press;.
     [Google Scholar]
  • Wang S. , Sun L. , Wei D. , Zhou B. , Zhang J. , Gu X. , Zhang L. , Liu Y. , Li Y. et al. ( 2014;). Bacillus daqingensis sp. nov., a halophilic, alkaliphilic bacterium isolated from saline-sodic soil in Daqing, China. . J Microbiol 52: 548––553. [CrossRef] [PubMed]
    [Google Scholar]
  • Zakrzewska-Czerwinska J. , Mordarski M. , Goodfellow M. . ( 1988;). DNA base composition and homology values in the classification of some rhodococcus species. . J Gen Microbiol 134: 2807––2813. [CrossRef] [PubMed]
    [Google Scholar]
  • Zhai L. , Liao T. , Xue Y. , Ma Y. . ( 2012;). Bacillus daliensis sp. nov., an alkaliphilic, gram-positive bacterium isolated from a soda lake. . Int J Syst Evol Microbiol 62: 949––953. [CrossRef] [PubMed]
    [Google Scholar]
  • Zhao B. , Wang H. , Li R. , Mao X. . ( 2010;). Thalassospira xianhensis sp. nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium. . Int J Syst Evol Microbiol 60: 1125––1129. [CrossRef] [PubMed]
    [Google Scholar]
  • Zhao B. , Chen S. . ( 2012;). Alkalitalea saponilacus gen. nov., sp. nov., an obligately anaerobic, alkaliphilic, xylanolytic bacterium from a meromictic soda lake. . Int J Syst Evol Microbiol 62: 2618––2623. [CrossRef] [PubMed]
    [Google Scholar]
  • Zhilina T. N. , Appel R. , Probian C. , Brossa E. L. , Harder J. , Widdel F. , Zavarzin G. A. . ( 2004;). Alkaliflexus imshenetskii gen. nov. sp. nov., a new alkaliphilic gliding carbohydrate-fermenting bacterium with propionate formation from a soda lake. . Arch Microbiol 182: 244––253. [CrossRef] [PubMed]
    [Google Scholar]
  • Zhu D. , Tanabe S. H. , Xie C. , Honda D. , Sun J. , Ai L. . ( 2014;). Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea. . Int J Syst Evol Microbiol 64: 1712––1717. [CrossRef] [PubMed]
    [Google Scholar]
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