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- Volume 66, Issue 8
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f Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis
- Authors: Kathryn A. Bernard1,2 , Ana Luisa Pacheco1 , Courtney Loomer1 , Tamara Burdz1 , Deborah Wiebe1 , Chris Huynh1 , Brynn Kaplen1 , Adam B. Olson1 , Margo Cnockaert3 , Hiroshi Eguchi4 , Tomomi Kuwahara5 , Haruyuki Nakayama-Imaohji5 , Hiroshi Shiota6 , Michael Boudewijns7 , Frederik Van Hoecke8 , Peter Vandamme3
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1 1National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada 2 2Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada 3 3Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium 4 4Department of Ophthalmology, Sakai Hospital, Kinki University Faculty of Medicine, Osaka, Japan 5 5Department of Microbiology, Faculty of Medicine, Kagawa University, Kagawa, Japan 6 6Department of Ophthalmology, Kaisei General Hospital, Kagawa, Japan 7 7Department of Microbiology, AZ Groeninge Hospital, Kortrijk, Belgium 8 8Department of Microbiology, Sint Andries Hospital of Tielt, Tielt, Belgium
- Correspondence Kathryn A. Bernard [email protected]
- First Published Online: 01 August 2016, International Journal of Systematic and Evolutionary Microbiology 66: 2803-2812, doi: 10.1099/ijsem.0.001059
- Subject: New taxa - Actinobacteria
- Received:
- Accepted:
- Cover date:




Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis, Page 1 of 1
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Strains of members of the genus Corynebacterium derived from ophthalmologic patients in Japan, Belgium and Switzerland and found to be closely related to-, but distinguishable from Corynebacterium mastitidis by 16S rRNA gene sequencing, were characterized using biochemical, chemotaxonomic, MALDI-TOF mass spectrometry and antimicrobial susceptibility methods and DNA–DNA hybridization as well as by whole-genome sequencing (WGS). Based on this investigation, we describe Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human ocular specimens, as well as emend the description of Corynebacterium mastitidis . Type strains for these species are: C. lowii R-50085T (=LMG 28276T =CCUG 65815T) and C. oculi R-50187T (=LMG 28277T =CCUG 65816T). DNA G+C content was found to be 62.2 % (by HPLC) and 62.8 % (by WGS) for C. lowii R-50085T, 64.1 % (HPLC) and 64.8 % (WGS) for C. oculi R-50187T and 67.8 % (HPLC) for C. mastitidis LMG 19040T [=S-8T =CCUG 38654T =CECT 4843T =CIP 105509T =DSM 44356T =IFO (NBRC)16160T =JCM 12269T].
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Genbank accession numbers derived from this study:16S rRNA gene sequences: KJ938704-KJ938714 (C. lowii R-50085T, KJ938705; C. oculi R-50187T, KJ938709) and rpoB sequences: KJ938692-KJ938702 (C. lowii R-50085T, KJ938693; C. oculi R-50187T, KJ938697). Whole-genome sequences for C. lowii R-50085T and C. oculi R-50187T have been deposited under the Genbank accession numbers LKEV00000000 and LKST00000000, respectively.
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Type strains have been deposited under accession numbers: C. lowii LMG 28276T and CCUG 65815T and C. oculi LMG 28277T and CCUG 65816T.
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One supplementary table is available with the online Supplementary Material.
- Keyword(s): Corynebacterium species recovered from eyes, emendation of Corynebacterium mastitidis description, Corynebacterium oculi, Corynebacterium lowii
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Abbreviations: ANI average nucleotide identity using BLASTN (ANIb) BHIB brain heart infusion broth BWA Burrows-Wheeler Aligner CAMP Christie, Atkins, Munch-Petersen assay CFA cellular fatty acids FAS fatty acid synthetase proteins FLASH fast length adjustment of short reads LMG Laboratory of Microbiology, University of Ghent MSP mass spectral profile NML National Microbiology Laboratory SPAdes ST. Petersburg genome assembler Sspace SSAKE-bases Scaffolding of Pre-Assembled Contigs after Extension TSI triple sugar iron agar slants WGS whole genome sequencing
© 2016 Her Majesty the Queen, in right of Canada, represented by the Canadian Minister of Health | Published by the Microbiology Society
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