Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis

Kathryn A. Bernard; Ana Luisa Pacheco; Courtney Loomer; Tamara Burdz; Deborah Wiebe; Chris Huynh; Brynn Kaplen; Adam B. Olson; Margo Cnockaert; Hiroshi Eguchi; Tomomi Kuwahara; Haruyuki Nakayama-Imaohji; Hiroshi Shiota; Michael Boudewijns; Frederik Van Hoecke; Peter Vandamme

doi:10.1099/ijsem.0.001059

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f Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis

Authors: Kathryn A. Bernard^1,2 , Ana Luisa Pacheco¹ , Courtney Loomer¹ , Tamara Burdz¹ , Deborah Wiebe¹ , Chris Huynh¹ , Brynn Kaplen¹ , Adam B. Olson¹ , Margo Cnockaert³ , Hiroshi Eguchi⁴ , Tomomi Kuwahara⁵ , Haruyuki Nakayama-Imaohji⁵ , Hiroshi Shiota⁶ , Michael Boudewijns⁷ , Frederik Van Hoecke⁸ , Peter Vandamme³
- VIEW AFFILIATIONS
- ¹ 1National Microbiology Laboratory-CSCHAH, Public Health Agency of Canada, Winnipeg, Manitoba, Canada ² 2Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada ³ 3Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium ⁴ 4Department of Ophthalmology, Sakai Hospital, Kinki University Faculty of Medicine, Osaka, Japan ⁵ 5Department of Microbiology, Faculty of Medicine, Kagawa University, Kagawa, Japan ⁶ 6Department of Ophthalmology, Kaisei General Hospital, Kagawa, Japan ⁷ 7Department of Microbiology, AZ Groeninge Hospital, Kortrijk, Belgium ⁸ 8Department of Microbiology, Sint Andries Hospital of Tielt, Tielt, Belgium
Correspondence Kathryn A. Bernard [email protected]
First Published Online: 01 August 2016, International Journal of Systematic and Evolutionary Microbiology 66: 2803-2812, doi: 10.1099/ijsem.0.001059
Subject: New taxa - Actinobacteria

Received: 29/10/2015
Accepted: 29/03/2016
Cover date: 01/08/2016

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Strains of members of the genus Corynebacterium derived from ophthalmologic patients in Japan, Belgium and Switzerland and found to be closely related to-, but distinguishable from Corynebacterium mastitidis by 16S rRNA gene sequencing, were characterized using biochemical, chemotaxonomic, MALDI-TOF mass spectrometry and antimicrobial susceptibility methods and DNA–DNA hybridization as well as by whole-genome sequencing (WGS). Based on this investigation, we describe Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human ocular specimens, as well as emend the description of Corynebacterium mastitidis . Type strains for these species are: C. lowii R-50085T (=LMG 28276T =CCUG 65815T) and C. oculi R-50187T (=LMG 28277T =CCUG 65816T). DNA G+C content was found to be 62.2 % (by HPLC) and 62.8 % (by WGS) for C. lowii R-50085T, 64.1 % (HPLC) and 64.8 % (WGS) for C. oculi R-50187T and 67.8 % (HPLC) for C. mastitidis LMG 19040T [=S-8T =CCUG 38654T =CECT 4843T =CIP 105509T =DSM 44356T =IFO (NBRC)16160T =JCM 12269T].

Genbank accession numbers derived from this study:16S rRNA gene sequences: KJ938704-KJ938714 (C. lowii R-50085T, KJ938705; C. oculi R-50187T, KJ938709) and rpoB sequences: KJ938692-KJ938702 (C. lowii R-50085T, KJ938693; C. oculi R-50187T, KJ938697). Whole-genome sequences for C. lowii R-50085T and C. oculi R-50187T have been deposited under the Genbank accession numbers LKEV00000000 and LKST00000000, respectively.
Type strains have been deposited under accession numbers: C. lowii LMG 28276T and CCUG 65815T and C. oculi LMG 28277T and CCUG 65816T.
One supplementary table is available with the online Supplementary Material.

Keyword(s): Corynebacterium species recovered from eyes, emendation of Corynebacterium mastitidis description, Corynebacterium oculi, Corynebacterium lowii

Abbreviations:

ANI	average nucleotide identity using BLASTN (ANIb)
BHIB	brain heart infusion broth
BWA	Burrows-Wheeler Aligner
CAMP	Christie, Atkins, Munch-Petersen assay
CFA	cellular fatty acids
FAS	fatty acid synthetase proteins
FLASH	fast length adjustment of short reads
LMG	Laboratory of Microbiology, University of Ghent
MSP	mass spectral profile
NML	National Microbiology Laboratory
SPAdes	ST. Petersburg genome assembler
Sspace	SSAKE-bases Scaffolding of Pre-Assembled Contigs after Extension
TSI	triple sugar iron agar slants
WGS	whole genome sequencing

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