%0 Journal Article %A Zanoni, Renato Giulio %A Piva, Silvia %A Florio, Daniela %A Bassi, Patrizia %A Mion, Domenico %A Cnockaert, Margo %A Luchetti, Andrea %A Vandamme, Peter %T Helicobacter apri sp. nov., isolated from wild boars %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 8 %P 2876-2882 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001071 %K wild boars %K Helicobacter sp. nov. %K polyphasic toxonomic study %I Microbiology Society, %X Three isolates (A19T, C21 and F12) with spiral-shaped cells and one bipolar sheathed flagellum were obtained from gastric mucosa and caecal contents of three different wild boars (Sus scrofa) and subjected to a polyphasic taxonomic study. A genus-specific PCR showed that these isolates belonged to the genus Helicobacter . Phylogenetic analysis based on 16S rRNA, 60-kDa heat-shock protein (HSP60) and atpA genes demonstrated they formed a novel lineage within this genus. Pairwise 16S rRNA, HSP60 and atpA gene sequence comparisons of the three isolates revealed 99.7, 99.4 and 99.9 % similarity, respectively, among the three isolates; the 16S rRNA gene of isolate A19T shared 98.5 % sequence similarity with its nearest validly named neighbouring species, Helicobacter mastomyrinus (to the type strain MIT 97-5577T). The taxonomic uniqueness of the wild boar isolates was confirmed by protein analysis performed by matrix-assisted laser desorption/ionization time-of-flight MS and by a distinctive biochemical profile. These data indicated that isolates A19T, C21 and F12 represent a novel taxon, for which the name Helicobacter apri sp. nov. is proposed, with isolate A19T (=DSM 28990T=LMG 28471T) as the type strain. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001071