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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 8

Reclassification of four strains as representatives of comb. nov., sp. nov., sp. nov. and sp. nov., and emended description of Open Access

Abstract

Genome comparisons based on average nucleotide identity (ANI) values of four strains currently classified as subsp. resulted in ANI values of 75.7–78.4 %, suggesting that each of those strains represents a separate species. The species . was proposed by Heckmann and Schmidt in 1987 to accommodate obligate endosymbionts of ciliates affiliated with the genus . The required revision of this species is, however, hampered by the fact, that this species is based only on a description and lacks a type strain available as pure culture. Furthermore, the ciliate culture ATCC 30859, on which the description of the species was based, is no longer available. We found another culture (Ammermann) sharing the same origin with ATCC 30859 and proved the identity of the endosymbionts contained in the two cultures. A multilocus sequence comparison approach was used to estimate if the four strains currently classified as subsp. share ANI values with the endosymbiont in the Ammermann culture above or below the threshold for species demarcation. A significant correlation (R 0.98, <0.0001) between multilocus sequence similarity and ANI values of genome-sequenced strains enabled the prediction that it is highly unlikely that these four strains belong to the species . . We propose reclassification of strains QLW-P1DMWA-1 (=DSM 18221=CIP 109841), MWH-MoK4 (=DSM 21495=CIP 110977), MWH-JaK3 (=DSM 21493=CIP 110976) and MWH-HuW1 (=DSM 21492=CIP 110978) as comb. nov., sp. nov., sp. nov. and sp. nov., respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ANI , average nucleotide identity , endosymbiont , New species , Polynucleobacter and revision
© 2016 IUMS
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2016-08-01
2024-04-24
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Reclassification of four Polynucleobacter necessarius strains as representatives of Polynucleobacter asymbioticus comb. nov., Polynucleobacter duraquae sp. nov., Polynucleobacter yangtzensis sp. nov. and Polynucleobacter sinensis sp. nov., and emended description of Polynucleobacter necessarius
Int J Syst Evol Microbiol 66, 2883 (2016); https://doi.org/10.1099/ijsem.0.001073
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