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Volume 66, Issue 7

sp. nov., isolated from the soil of a cave Free

Abstract

A Gram-reaction-positive, strictly aerobic, capsule-forming, motile and rod-shaped bacterium, designated strain XD80, was isolated from the soil of a native cave in Lichuan, Hubei province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XD80 was most closely related to CCTCC AB 2015183 (98.66 % sequence similarity), followed by DSM 25058(97.83 %), KCTC 33549 (97.70 %), CCTCC AB 2011121 (97.21 %) and DSM 28032 (96.96 %). Levels of DNA–DNA relatedness between strain XD80 and CCTCC AB 2015183, DSM 25058, KCTC 33549 and CCTCC AB 2011121 were 37.4 %, 53.8 %, 53.6 % and 52.7 %, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipid and two unknown phospholipids. The predominant fatty acids (>5 %) were iso-C (37.3 %), anteiso-C (10.8 %), C (10.4 %), iso-C (10.3 %), Cω11 (9.6 %) and anteiso-C (7.4 %). MK-6 (86.4 %) was the major respiratory quinone. The DNA G+C content was 46.4 mol%. The cell-wall peptidoglycan contained -diaminopimelic acid (type A1γ). Ribose and glucose were the major whole-cell sugars. In addition, strain XD80 showed differential physiological characteristics from most members of the genus , encompassing hydrolysis of starch, acid production from inositol and raffinose, and production of valine arylamidase. The results of this polyphasic study indicated that strain XD80 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XD80(=CCTCC AB 2015053=KCTC 33636).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Domibacillus soil Firmicutes Polyphasic identification
© 2016 IUMS
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2016-07-01
2024-04-25
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Domibacillus antri sp. nov., isolated from the soil of a cave
Int J Syst Evol Microbiol 66, 2502 (2016); https://doi.org/10.1099/ijsem.0.001080
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