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f Filimonas zeae sp. nov., an endophytic bacterium isolated from maize root

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  • Authors: Jun-Lian Gao1​ ,† ,  Pengbo Sun2​ ,† ,  Xu-Ming Wang1​ ,  Tian-Lei Qiu1​ ,  Fan-Yang Lv3​ ,  Ming-Ming Yang1​ ,  Mengzhu Lu2​ , Jian-Guang Sun3​
    • VIEW AFFILIATIONS
    • 1​ 1​ Beijing Agro- Biotechnology Research Center, Beijing Academy of Agriculture and Forestry/Beijing Municipal Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, PR China 2​ 2​ State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, PR China 3​ 3​ Key Laboratory of Microbial Resources , Ministry of Agriculture / Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China
  • Correspondence Jian-Guang Sun [email protected]
  • First Published Online: 01 July 2016, International Journal of Systematic and Evolutionary Microbiology 66: 2730-2734, doi: 10.1099/ijsem.0.001116
  • Subject: New taxa - Bacteroidetes
  • Received:
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Filimonas zeae sp. nov., an endophytic bacterium isolated from maize root, Page 1 of 1

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  • A novel Gram-stain-negative, aerobic, motile by gliding and filamentous strain, designated 772,was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, China. 16S rRNA gene sequence analysis indicated that strain 772 was closely related to Filimonas endophytica SR2-06 and Filimonas lacunae YT21of the family Chitinophagaceae with sequence similarities of 99.0 and 96.9 %, respectively. However, the new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to Filimonas. endophytica KCTC 42060 (18.7±1.8 %) and Filimonas. lacunae DSM 21054(17.9±2.0%). The DNA G+C content of strain 772 was 44.9 mol%. The respiratory quinone was menaquinone-7 and the polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and one unidentified lipid. The major fatty acids were iso-C15 : 0 and iso-C15 : 1 G. The results of the physiological and biochemical tests and minor differences in the fatty acid profiles allowed the clear phenotypic differentiation of strain 772 from the closely related species Filimonas. endophytica andF. lacunae. Strain 772thus represents a novel species within the genus Filimonas , for which the name Filimonas zeae sp. nov. is proposed. The type strain is 772 (=CGMCC 1.15290=DSM 100760).

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDJB accession number for the 16S rRNA gene sequence of strain 772 is KR610522.

  • Two supplementary figures are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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