RT Journal Article SR Electronic(1) A1 Song, Hye Seon A1 Cha, In-Tae A1 Rhee, Jin-Kyu A1 Yim, Kyung June A1 Kim, Ah Yoon A1 Choi, Jong-Soon A1 Baek, Su Jeong A1 Seo, Myung-Ji A1 Park, Soo-Je A1 Nam, Young-Do A1 Roh, Seong WoonYR 2016 T1 Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 7 SP 2740 OP 2746 DO https://doi.org/10.1099/ijsem.0.001118 PB Microbiology Society, SN 1466-5034, AB A novel halophilic archaeon designated strain CBA1114T was isolated from solar salt in the Republic of Korea. Strain CBA1114T, cells of which were coccoid and Gram-stain-negative, grew in the presence of 15–30 % (w/v) NaCl (optimum, 20 %) and at 20–50 °C (optimum, 40 °C) and pH 7.0–9.0 (optimum, pH 8.0). Strain CBA1114T required Mg2+ for growth. Strain CBA1114T had three 16S rRNA genes, rrnA, rrnB and rrnC; levels of similarity between the sequences were 99.7–99.9 %. The 16S rRNA gene sequence of strain CBA1114T showed 91.7 % similarity to that of Haloterrigena thermotolerans PR5T. In multilocus sequence analysis (MLSA), five housekeeping genes, atpB, EF-2, radA, rpoB′ and secY, were found to be closely related to those of the members of the genera Halorientalis (89.7 % similarity of the atpB gene sequence), Halomicroarcula (91.9 %, EF-2), Haloterrigena (85.4 %, radA), Natronoarchaeum (89.2 %, rpoB′) and Natrinema (75.7 %, secY). A phylogenetic tree generated from the results of MLSA of the five housekeeping genes showed that strain CBA1114T was closely related to species of the genus Halorientalis in the family Halobacteriaceae . The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and unidentified lipids. The G+C content of the genomic DNA of strain CBA1114T was 68.1 mol%. According to the results of phylogenetic, phenotypic and chemotaxonomic analyses, we designate strain CBA1114T (=JCM 30111T=KCTC 4206T) as the type strain of Halostella salina gen. nov., sp. nov., a novel species of a new genus within the family Halobacteriaceae ., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001118