RT Journal Article SR Electronic(1) A1 Bell, Melissa E. A1 Bernard, Kathryn A. A1 Harrington, Susan M. A1 Patel, Nisha B. A1 Tucker, Trudy-Ann A1 Metcalfe, Maureen G. A1 McQuiston, John R.YR 2016 T1 Lawsonella clevelandensis gen. nov., sp. nov., a new member of the suborder Corynebacterineae isolated from human abscesses JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 8 SP 2929 OP 2935 DO https://doi.org/10.1099/ijsem.0.001122 PB Microbiology Society, SN 1466-5034, AB Gram-stain-positive, partially acid-fast, non-spore-forming, anaerobic, catalase-positive, pleomorphic bacteria were isolated from human abscesses. Strains X1036T, X1698 and NML 120705, were recovered from a spinal abscess, a peritoneal abscess and a breast abscess respectively. A phylogenetic analysis of the 16S rRNA gene sequences showed that the strains shared 100 % similarity, and the nearest phylogenetic neighbour was Dietzia timorensis DSM 45568T (95%). Chemotaxonomic characteristics of the strains were consistent with those described for members of the suborder Corynebacterineae. Mycolic acids were detected using HPLC and one-dimensional TLC; whole-cell hydrolysates yielded meso-diaminopimelic acid with arabinose and galactose as the predominant sugars; the muramic acid acyl type was acetylated; the major menaquinone was MK-9 (96.3%); polar lipids detected were phosphatidylglycerol, phosphatidylinositol and an unknown glycophospholipid. Cellular fatty acids were hexadecanoic acid (C16 : 0), octadecenoic acid (C18 : 1ω9c) and decanoic acid (C10 : 0). Tuberculostearic acid was not detected. Based on the results of this polyphasic study, we conclude that these strains represent a novel genus and species within the suborder Corynebacterineae for which we propose the name Lawsonella clevelandensis gen. nov., sp. nov., with the type strain X1036T (=DSM 45743T=CCUG 66657T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001122