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Volume 66, Issue 8

sp. nov., isolated from a tidal flat Free

Abstract

A Gram-stain-negative, non-spore-forming, non-motile and coccoid, ovoid or short-rod-shaped bacterial strain, designated GHD-30, was isolated from a tidal flat on the Yellow Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain GHD-30 grew optimally at 30 °C, at pH 6.5–7.5 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain GHD-30represented a member of the genus . Strain GHD-30 exhibited 16S rRNA gene sequence similarity values of 97.10–97.33 % to the type strains of , and and of 94.35–96.97 % to the type strains of the other species of the genus . Strain GHD-30 contained Q-10 as the predominant ubiquinone and Cω7 as the major fatty acid. The major polar lipids detected in strain GHD-30 were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified aminolipid. The DNA G+C content of strain GHD-30 was 62.0 mol% and its mean DNA–DNA relatedness values with the type strains of and were 11–19 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHD-30 is separated from recognized species of the genus . On the basis of the data presented, strain GHD-30 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GHD-30 (=KCTC 52214 =NBRC 111993).

  • Received:
  • Accepted:
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Keyword(s): Novel species , Paracoccus aestuariivivens sp. nov. , Polyphasic taxonomy and tidal flat
© 2016 IUMS
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2016-08-01
2024-04-19
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References

  1. Anzai Y., Kim H., Park J. Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [View Article][PubMed]
     [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef]
     [Google Scholar]
  3. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In The Prokaryotes pp. 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  5. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 49:25–68 [View Article]
     [Google Scholar]
  6. Davis D. H., Doudoroff M., Stanier R. Y., Mandel M. 1969; Proposal to reject the genus Hydrogenomonas: Taxonomic implications. Int J Syst Bacteriol 19:375–390 [View Article]
     [Google Scholar]
  7. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics pp. 121–161 Edited by Goodfellow M., O’Donnell A. G. Chichester: John Wiley & Sons;
     [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
     [Google Scholar]
  9. Jung Y.-T., Park S., Lee J.-S., Yoon J.-H. 2014; Paracoccus lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64:2763–2769 [View Article]
     [Google Scholar]
  10. Kelly D. P., Rainey F. A., Wood A. P. 2006; The genus Paracoccus . In The Prokaryotes, 3rd edn. vol. 5 pp. 232–249 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer; [CrossRef]
     [Google Scholar]
  11. Khan S. T., Takaichi S., Harayama S. 2008; Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay. Int J Syst Evol Microbiol 58:383–386 [View Article][PubMed]
     [Google Scholar]
  12. Kim Y. O., Kong H. J., Park S., Kang S. J., Kim K. K., Moon D. Y., Oh T. K., Yoon J. H. 2010; Paracoccus fistulariae sp. nov., a lipolytic bacterium isolated from bluespotted cornetfish, Fistularia commersonii . Int J Syst Evol Microbiol 60:2908–2912 [View Article][PubMed]
     [Google Scholar]
  13. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
     [Google Scholar]
  14. Lee K. B., Liu C. T., Anzai Y., Kim H., Aono T., Oyaizu H. 2005; The hierarchical system of the ‘Alphaproteobacteria': description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55:1907–1919 [View Article][PubMed]
     [Google Scholar]
  15. Lee M.-J., Lee S.-S. 2013; Paracoccus limosus sp. nov., isolated from activated sludge in a sewage treatment plant. Int J Syst Evol Microbiol 53:1311–1316 [CrossRef]
     [Google Scholar]
  16. Li H. F., Qu J. H., Yang J. S., Li Z. J., Yuan H. L. 2009; Paracoccus chinensis sp. nov., isolated from sediment of a reservoir. Int J Syst Evol Microbiol 59:2670–2674 [View Article][PubMed]
     [Google Scholar]
  17. Liu X. Y., Wang B. J., Jiang C. Y., Liu S. J. 2006; Paracoccus sulfuroxidans sp. nov., a sulfur oxidizer from activated sludge. Int J Syst Evol Microbiol 56:2693–2695 [View Article][PubMed]
     [Google Scholar]
  18. Liu Z. P., Wang B. J., Liu X. Y., Dai X., Liu Y. H., Liu S. J. 2008; Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969. Int J Syst Evol Microbiol 58:257–261 [View Article][PubMed]
     [Google Scholar]
  19. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
     [Google Scholar]
  20. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  21. Nguyen N. L., Kim Y. J., Hoang V. A., Tran B. T., Pham H. S., Yang D. C. 2015; Paracoccus panacisoli sp. nov., isolated from a forest soil cultivated with Vietnamese ginseng. Int J Syst Evol Microbiol 65:1491–1497 [View Article][PubMed]
     [Google Scholar]
  22. Nokhal T.-H., Schlegel H. G. 1983; Taxonomic study of Paracoccus denitrificans . Int J Syst Bacteriol 33:26–37 [View Article]
     [Google Scholar]
  23. Park S., Park D. S., Bae K. S., Yoon J. H. 2014; Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 64:1378–1383 [View Article][PubMed]
     [Google Scholar]
  24. Parte A. C. 2014; LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42:D613–D616 [View Article][PubMed]
     [Google Scholar]
  25. Roh S. W., Nam Y. D., Chang H. W., Kim K. H., Kim M. S., Shin K. S., Yoon J. H., Oh H. M., Bae J. W. 2009; Paracoccus aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 59:790–794 [View Article][PubMed]
     [Google Scholar]
  26. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI. Inc;
     [Google Scholar]
  27. Sheu S. Y., Jiang S. R., Chen C. A., Wang J. T., Chen W. M. 2011; Paracoccus stylophorae sp. nov., isolated from the reef-building coral Stylophora pistillata . Int J Syst Evol Microbiol 61:2221–2226 [View Article][PubMed]
     [Google Scholar]
  28. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
     [Google Scholar]
  29. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
     [Google Scholar]
  30. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International committee on systematic bacteriology. report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  32. Yoon J. H., Lee S. T., Park Y. H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
     [Google Scholar]
  33. Yoon J. H., Kang K. H., Park Y. H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [View Article][PubMed]
     [Google Scholar]
  34. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
     [Google Scholar]
  35. Zhang G., Yang Y., Yin X., Wang S. 2014; Paracoccus pacificus sp. nov., isolated from the Western Pacific Ocean. Antonie Van Leeuwenhoek 106:725–731 [View Article][PubMed]
     [Google Scholar]
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Paracoccus aestuariivivens sp. nov., isolated from a tidal flat
Int J Syst Evol Microbiol 66, 2992 (2016); https://doi.org/10.1099/ijsem.0.001133
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