%0 Journal Article %A Dunlap, Christopher A. %A Saunders, Lauren P. %A Schisler, David A. %A Leathers, Timothy D. %A Naeem, Naveed %A Cohan, Frederick M. %A Rooney, Alejandro P. %T Bacillus nakamurai sp. nov., a black-pigment-producing strain %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 8 %P 2987-2991 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001135 %K Pigment %K black %I Microbiology Society, %X Two isolates of a Gram-stain-positive, strictly aerobic, motile, rod-shaped, endospore-forming bacterium were identified during a survey of the Bacillus diversity of the Agriculture Research Service Culture Collection. These strains were originally isolated from soil and have a phenotype of producing a dark pigment on tryptic soy agar. Phylogenetic analysis of the 16S rRNA gene indicated that these strains were related most closely to Bacillus subtilis subsp. inaquosorum (99.7 % similarity) and Bacillus axarquiensis (99.7 %). In phenotypic characterization, the novel strains were found to grow between 17 and 50 °C and can tolerate up to 9 % (w/v) NaCl. Furthermore, the strains grew in media of pH 5.5–10 (optimal growth at pH 7.0–8.0). The predominant cellular fatty acids were anteiso-C15 : 0 (34.8 %) and iso-C15 : 0 (21.9 %). The cell-wall peptidoglycan contained meso-diaminopimelic acid. A draft genome of both strains was completed. The DNA G+C content was 43.8 mol%. A phylogenomic analysis on the core genome of these two new strains and all members of the Bacillus subtilis group revealed these two strains formed a distinct monophyletic clade with the nearest neighbour Bacillus amyloliquefaciens . DNA–DNA relatedness studies using in silico DNA–DNA hybridizations showed the two strains were conspecific (93.8 %), while values with all other species (<31.5 %) were well below the species threshold of 70 %. Based on the consensus of phylogenetic and phenotypic analyses, these strains are considered to represent a novel species within the genus Bacillus , for which the name Bacillus nakamurai sp. nov. is proposed, with type strain NRRL B-41091T (=CCUG 68786T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001135