%0 Journal Article %A Lin, Shih-Yao %A Wu, Yun-Hsuan %A Hameed, Asif %A Liu, You-Cheng %A Young, Chiu-Chung %T Ammoniphilus resinae sp. nov., an endospore-forming bacterium isolated from resin fragments %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 8 %P 3010-3016 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001137 %K Ammoniphilus oxalaticus %K Ammoniphilus oxalivorans %K Strain CC-RT-ET %I Microbiology Society, %X A polyphasic approach was used to characterize an endospore-forming bacterium, designated strain CC-RT-ET, isolated from resin fragments. Strain CC-RT-ET was Gram-stain-variable and facultatively anaerobic, able to grow at 20–37 °C (optimal 30 °C) and pH6.0–9.0 (optimal pH 7.0). Ellipsoidal and terminal endospores were observed. The 16S rRNA gene sequence analysis of strain CC-RT-ET showed highest sequence similarity to Ammoniphilus oxalivorans RAOx-FST (97.8 %) and Ammoniphilus oxalaticus RAOx-1T (97.7 %). According to the DNA–DNA hybridization, the relatedness values of strain CC-RT-ET with Ammoniphilus oxalivorans RAOx-FST and Ammoniphilus oxalaticus RAOx-1T were 21 % and 29 %, respectively. The DNA G+C content was 39.2 mol% and the predominant quinone system was menaquinone-7 (MK-7). The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polyamine pattern showed predominance of sym-homospermidine and putrescine. The major fatty acids found in strain CC-RT-ET were C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and C16 : 1 ω7c/C16 : 1 ω6c. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain CC-RT-ET is considered to represent a novel species of the genus Ammoniphilus , for which the name Ammoniphilus resinaesp. nov. is proposed. The type strain is CC-RT-ET (=BCRC 80314T=DSM 24738T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001137