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f Paraphotobacterium marinum gen. nov., sp. nov., a member of the family Vibrionaceae, isolated from surface seawater

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  • Authors: Zhaobin Huang1​ ,2​ ,3​ ,  Chunming Dong1​ ,2​ ,3​ , Zongze Shao1​ ,2​ ,3​
    • VIEW AFFILIATIONS
    • 1​ 1​ State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China 2​ 2​ Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China 3​ 3​ Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, P. R. China
  • Correspondence Zongze Shao [email protected]
  • First Published Online: 01 August 2016, International Journal of Systematic and Evolutionary Microbiology 66: 3050-3056, doi: 10.1099/ijsem.0.001142
  • Subject: New taxa - Proteobacteria
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  • A novel Gram-stain-negative bacterium, named strain NSCS20N07D, was isolated from surface seawater of the South China Sea. Cells of this strain contained poly-β-hydroxybutyrate granules. Growth was observed at 15–35 °C with optimum of 30 °C, at a salinity range of 1–6 % (w/v) NaCl with optimum of 3 % and at pH 5–8 with optimum of pH 5. The full-length 16S rRNA gene sequence of strain NSCS20N07D showed highest similarity to Photobacterium iliopiscarium ATCC 51760 of 96.0 %, followed by Photobacterium kishitanii pjapo.1.1, Photobacterium phosphoreum ATCC 11040 and Photobacterium aquimaris LC2-065 of 96.0, 95.8 and 95.7 %, respectively. Phylogenetic analysis showed that strain NSCS20N07D formed a separate clade distinct from species of the genus Photobacterium and other genera within the family Vibrionaceae , indicating that strain NSCS20N07D represented a novel genus affiliated with this family. The genome size of strain NSCS20N07D was 2.5 Mb, which was much smaller than those of related species in the family Vibrionaceae . The predominant fatty acids were C16 : 0, C17 : 0 cyclo, C19 : 0 cyclo ω8c, C18 : 0 and summed feature 2 (C14 : 0 3-OH/iso-C16 : 1 I). The respiratory quinone was Q-8. The polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and four unidentified lipids. The DNA G+C content was 30.7 mol%. Combined, these results suggest that strain NSCS20N07D represents a novel species of a new genus, for which the name Paraphotobacterium marinum gen. nov., sp. nov. is proposed. The type strain of Paraphotobacterium marinum is NSCS20N07D(=KCTC 52126=MCCC 1A01886=CIP 111031).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Paraphotobacterium marinum strain NSCS20N07D is KU140414.

  • Six supplementary figures are available with the online Supplementary Material.

  • Abbreviations:
    PHB poly-β-hydroxybutyrate

© 2016 IUMS | Published by the Microbiology Society

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2019-02-22
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