RT Journal Article SR Electronic(1) A1 Yokota, Akira A1 Ningsih, Fitria A1 Nurlaili, Dafina Ghossani A1 Sakai, Yasuteru A1 Yabe, Shuhei A1 Oetari, Ariyanti A1 Santoso, Iman A1 Sjamsuridzal, WellyzarYR 2016 T1 Paenibacillus cisolokensis sp. nov., isolated from litter of a geyser JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 8 SP 3088 OP 3094 DO https://doi.org/10.1099/ijsem.0.001151 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, endospore-forming, aerobic and thermophilic bacterium, designated strain LC2-13AT, was isolated from Cisolok geyser, West Java, Indonesia, at 50 °C. The isolate was rod-shaped and motile by means of peritrichous flagella. The major cellular fatty acids were iso-C16 : 0, C16 : 0 and anteiso-C15 : 0 and the major quinone was menaquinone 7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was 56.6 mol% and the major diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain LC2-13AT is related most closely to Paenibacillus kobensis DSM 10249T (94.86 % similarity), Paenibacillus tarimensis SA-7-6T (94.77 %) and Paenibacillus barengoltzii SAFN-016T (94.77 %). On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain LC2-13AT is affiliated to the genus Paenibacillus , but could be distinguished from recognized species of this genus. A novel species with the name Paenibacillus cisolokensis sp. nov. is thus proposed. The type strain is LC2-13AT (=UICC B-42T=NRRL B-65368T=DSM 101873T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001151