1887

Abstract

Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of ATCC 13950. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (,,, and) revealed the phylogenetic separation of these strains from ATCC 13950. DNA–DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of and confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the complex named . The type strain is MOTT64 (=KCTC 29084=JCM 30622).

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2016-08-01
2024-03-28
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