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Volume 66, Issue 8

sp. nov., from oil-contaminated soil Free

Abstract

During the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Gunsan, North Jeolla Province, South Korea, a light-grey-pigmented, Gram-staining-negative, aerobic, non-motile and rod-shaped bacterium, designated strain D39, was isolated. This strain was non-sporulating, catalase-negative and oxidase-positive. It was able to grow at 12–42 °C, pH 5.5–8.5 and with 0–1 % (w/v) NaCl. This strain was characterized taxonomically by a polyphasic approach. Based on the results of 16S rRNA gene sequence analysis, strain D39 belongs to the genus and is closely related to ‘' FW-6 (97.30 % sequence similarity), SM117 (97.17 % sequence similarity) and E-II-3 (97.01 % sequence similarity). The only respiratory quinone was ubiquinone-10 and the major polyamine was spermidine. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and phosphatidyl--methylethanolamine. The predominant fatty acids of strain D39 were summed feature 8 (Cω7 and/or Cω6), summed feature 3 (Cω7 and/or Cω6), Cω6 and C 2-OH. The genomic DNA G+C content of this novel strain was 66.7 mol%. The DNA–DNA relatedness between strain D39 and ‘' KACC 16615, KACC 14598, KACC 17599 and DSM 25975 were 33.7 %, 29.0 %, 22.3 % and 18.3 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D39 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D39 (=KEMB 9005-346=KACC 18593=JCM 31158).

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Keyword(s): Novosphingobium naphthae sp. nov. , oil-contaminated soil , Proteobacteria and Sphingomonadaceae
© 2016 IUMS
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2016-08-01
2024-04-25
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Novosphingobium naphthae sp. nov., from oil-contaminated soil
Int J Syst Evol Microbiol 66, 3170 (2016); https://doi.org/10.1099/ijsem.0.001164
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