RT Journal Article SR Electronic(1) A1 Chaudhary, Dhiraj Kumar A1 Kim, JaisooYR 2016 T1 Novosphingobium naphthae sp. nov., from oil-contaminated soil JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 8 SP 3170 OP 3176 DO https://doi.org/10.1099/ijsem.0.001164 PB Microbiology Society, SN 1466-5034, AB During the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Gunsan, North Jeolla Province, South Korea, a light-grey-pigmented, Gram-staining-negative, aerobic, non-motile and rod-shaped bacterium, designated strain D39T, was isolated. This strain was non-sporulating, catalase-negative and oxidase-positive. It was able to grow at 12–42 °C, pH 5.5–8.5 and with 0–1 % (w/v) NaCl. This strain was characterized taxonomically by a polyphasic approach. Based on the results of 16S rRNA gene sequence analysis, strain D39T belongs to the genus Novosphingobium and is closely related to ‘ Novosphingobium ginsenosidimutans' FW-6 (97.30 % sequence similarity), Novosphingobium mathurense SM117T (97.17 % sequence similarity) and Novosphingobium aquiterrae E-II-3T (97.01 % sequence similarity). The only respiratory quinone was ubiquinone-10 and the major polyamine was spermidine. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D39T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω6c and C14 : 0 2-OH. The genomic DNA G+C content of this novel strain was 66.7 mol%. The DNA–DNA relatedness between strain D39T and ‘N. ginsenosidimutans' KACC 16615, N. mathurense KACC 14598T, N. aquiterrae KACC 17599T and Novosphingobium kunmingense DSM 25975T were 33.7 %, 29.0 %, 22.3 % and 18.3 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D39T represents a novel species of the genus Novosphingobium , for which the name Novosphingobium naphthae sp. nov. is proposed. The type strain is D39T (=KEMB 9005-346T=KACC 18593T=JCM 31158T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001164