%0 Journal Article %A Matsumoto, Takehisa %A Negishi, Tatsuya %A Hamada, Moriyuki %A Komaki, Hisayuki %A Gonoi, Tohru %A Yaguchi, Takashi %T Nocardia shinanonensis sp. nov., isolated from a patient with endophthalmitis %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 9 %P 3324-3328 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001197 %K Nocardia shinanonensis %K 16S rRNA gene %K gyrB gene %K endophthalmitis %K Japanese patient %I Microbiology Society, %X A nocardioform strain IFM 11456T was isolated from the aqueous humor from a patient with endophthalmitis and was characterized to its taxonomic position. IFM 11456T contained arabinose, galactose and meso-diaminopimelic acid in whole-cell hydrolysates and mycolic acids that co-migrated with those from the type strain of Nocardia asteroides . The acyl type of muramic acid was N-glycolyl. The diagnostic polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and two unidentified glycolipids and the predominant menaquinone was MK-8(H4, ω-cycl.). These characteristics are typical of members of the genus Nocardia . Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate represented a novel species of the genus Nocardia and was most closely related to the type strains of Nocardia mikamii JCM 15508T (98.1 %) and Nocardia aobensis IFM 0372T (98.1 %). However, analysis of partial gyrB sequences showed that strain IFM 11456T had 90.2 % similarity to Nocardia concava IFM 0354T and 90 % to Nocardia niigatensis IFM 0330T. The DNA–DNA relatedness values for strain IFM 11456T compared with N. mikamii JCM 15508T, N. aobensis IFM 0372T and N. concava IFM 0354T ranged from 24.4 to 39.9 %. Phenotypic characteristics that differentiated IFM 11456T from phylogenetically related species were growth at 45 °C, utilization of citrate and growth with inositol as a sole carbon source. On the basis of this polyphasic study, the isolate represents a novel species within the genus Nocardia , for which the name Nocardia shinanonensis sp. nov. is proposed. The type strain is IFM 11456T (=NBRC 109590T=TBRC 5149T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001197