@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001204, author = "Aubin, Guillaume Ghislain and Bémer, Pascale and Kambarev, Stanimir and Patel, Nisha B. and Lemenand, Olivier and Caillon, Jocelyne and Lawson, Paul A. and Corvec, Stéphane", title = "Propionibacterium namnetense sp. nov., isolated from a human bone infection", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "9", pages = "3393-3399", doi = "https://doi.org/10.1099/ijsem.0.001204", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001204", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "bone", keywords = "infection", keywords = "16S rDNA sequence, rpoB, gyrB, recA", keywords = "Propionibacterium namnetense", abstract = "A polyphasic taxonomic study was performed on two Gram-positive-staining, anaerobic, pleomorphic, rod-shaped strains isolated from human bone and tissue samples. Sequencing of the 16S rRNA genes revealed that the strains belong to a novel species within the genus Propionibacterium , most closely related to Propionibacterium acnes subsp. acnes and Propionibacterium acnes subsp. elongatum with similarity values of 98.4 % and 98.1 %, respectively. In addition, protein-coding genes for rpoB, recA and gyrB clearly separated the novel organism from all species and subspecies of the genus Propionibacterium . However, a DNA–DNA hybridization analysis between the novel organism and the type strain P. acnes ATCC 6919T revealed a value of only 61.1 %. Furthermore, whole genome analysis using the program OrthoANI gave a value of 88.5 %, which is significantly below the cut-off value of 95 % for species delineation. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0. The DNA G+C content of the type strain was 59.7 mol%. When taken collectively, phenotypic, molecular genetic, chemotaxonomic and phylogenetic information demonstrate that the organism represents a distinct, albeit close relative of P. acnes On the basis of the results presented, the organism represents a novel member of the genus Propionibacterium for which the name Propionibacterium namnetense sp. nov. is proposed. The type strain is NTS 31307302T (=DSM 29427T=CCUG 66358T).", }