@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001258, author = "Huang, Huiqin and Feng, Fengzhao and Liu, Min and Zhang, Fute and Sun, Qianguang and Qin, Songyu and Bao, Shixiang", title = "Paenibacillus segetis sp. nov., isolated from soil of a tropical rainforest", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "9", pages = "3703-3707", doi = "https://doi.org/10.1099/ijsem.0.001258", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001258", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Paenibacillus segetis sp. nov.", keywords = "16S rRNA", keywords = "polyphasic taxonomy", abstract = "A Gram-stain-positive, facultatively anaerobic, endospore-forming, irregular rod-shaped bacterium, designated DB13260T, was isolated from tropical rainforest soil in Jianfengling Nature Reserve in Hainan, China. The isolate was found to grow with 0–4 % (w/v) NaCl, at 5–40 °C and pH 6.0–10.5, with an optimum of 0 % NaCl, 30–37 °C and pH 8.5–9.0, respectively. The predominant isoprenoid quinone was menaquinone 7 (MK-7), and the major fatty acids were anteiso-C15 : 0, iso-C16 : 0 and C16 : 0. The G+C content of the genomic DNA was 53.7 mol%. Analysis of the 16S rRNA gene sequence of strain DB13260T showed an affiliation of the strain with the genus P aenibacillus , sharing 98.3 % and 97.8 % 16S rRNA gene sequence similarities with the closest relatives P aenibacillus anaericanus MH21T and Paenibacillus selenii W126T, respectively. The DNA–DNA hybridization values between strain DB13260T and the two type strains were 60.4 % and 42.6 %, respectively. The combined phenotypic and DNA–DNA hybridization data supported the conclusion that strain DB13260T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus segetis sp. nov. is proposed. The type strain is DB13260T (=CGMCC 1.12769T=DSM 28014T).", }