RT Journal Article SR Electronic(1) A1 Muangham, Supattra A1 Suksaard, Paweena A1 Mingma, Ratchanee A1 Matsumoto, Atsuko A1 Takahashi, Yōko A1 Duangmal, KannikaYR 2016 T1 Nocardiopsissediminis sp. nov., isolated from mangrove sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 10 SP 3835 OP 3840 DO https://doi.org/10.1099/ijsem.0.001273 PB Microbiology Society, SN 1466-5034, AB A filamentous actinomycete, designated strain 1SS5-02T, was isolated from mangrove sediment collected from Ranong province, Thailand. The strain formed aerial and substrate mycelia composed of long, branched hyphae. Aerial mycelia differentiated into non-motile, rod-shaped spores. The organism contained meso-diaminopimelic acid and no diagnostic sugars in whole-cell hydrolysates. The predominant menaquinones were MK-11(H4), MK-11(H6) and MK-11(H8). Polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and four unidentified lipids. The major fatty acids were iso-C16 : 0, C18 : 1 ω9c, 10-methyl C18 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 73.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1SS5-02T belonged to the genus Nocardiopsis . The strain showed the highest degree of 16S rRNA gene sequence similarity with ‘ Nocardiopsis mangrovei ’ HA11166 (97.9 %) and Nocardiopsis trehalosi VKM Ac-942T (97.8 %). However, strain 1SS5-02T could be distinguished from its nearest phylogenetic relatives in the genus Nocardiopsis on the basis of DNA–DNA relatedness values and the combination of phenotypic properties. On the basis of polyphasic taxonomy, strain 1SS5-02T is considered to represent a novel species of the genus Nocardiopsis , for which the name Nocardiopsis sediminis sp. nov. is proposed. The type strain is 1SS5-02T (=BCC 75410T=NBRC 110934T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001273