%0 Journal Article %A Tsang, Chi-Ching %A Xiong, Lifeng %A Poon, Rosana W. S. %A Chen, Jonathan H. K. %A Leung, Kit-Wah %A Lam, Jimmy Y. W. %A Wu, Alan K. L. %A Chan, Jasper F. W. %A Lau, Susanna K. P. %A Woo, Patrick C. Y. %T Gordonia hongkongensis sp. nov., isolated from blood culture and peritoneal dialysis effluent of patients in Hong Kong %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 10 %P 3942-3950 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001292 %K hongkongensis %K novel %K Gordonia %K blood %K peritoneal dialysis effluent %I Microbiology Society, %X Two bacterial strains, HKU50T and HKU46, were isolated in Hong Kong from the blood culture and the peritoneal dialysis effluent of two patients. The strains are Gram-stain-positive, acid-fast, non-motile, non-sporulating bacilli. They grow on Columbia agar with 5 % defibrinated sheep blood and brain–heart infusion agar under aerobic conditions with 5 % CO2 at 37 °C as pink-to-orange, non-haemolytic colonies. The strains are catalase-positive and oxidase-negative, and have a unique biochemical profile distinguishable from other closely related species. DNA sequencing revealed that both isolates possessed multiple intra-genomic 16S rRNA gene copies (99.8–100 % sequence identities to Gordonia lacunae NRRL B-24551T and Gordonia terrae NRRL B-16283T). Phylogenetic analysis of the 16S rRNA gene, secA1 and gyrB showed that the two isolates formed a distinct branch within the genus Gordonia and were most closely related to G. lacunae and G. terrae . DNA–DNA hybridization demonstrated ≤53.7 % and ≤49.4 % DNA relatedness between the two isolates and G. lacunae , and between the two isolates and G. terrae , respectively. Hierarchical cluster analysis of MALDI–TOF MS main spectrum profiles showed that strains HKU50T and HKU46 were closely related to each other, but were distinct from G. lacunae , G. terrae , or any other species of the genus Gordonia in the Bruker database. The chemotaxonomic traits of the two strains were highly similar, and the major fatty acids were summed feature 4 (iso-C15 : 0 2-OH/C16 : 1 trans-9), C16 : 0, C18 : 1 cis-9, and tuberculostearic acid. A novel species named Gordonia hongkongensis sp. nov. is proposed to accommodate strains HKU50T and HKU46, with strain HKU50T (=CCOS 955T=CIP 111027T=NBRC 111234T=NCCP 16210T) as the type strain. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001292