Sphingomonas prati sp. nov., isolated from alpine meadow soil Manandhar, Pooja and Zhang, Gengxin and Hu, Yilun and Lama, Arun and Gao, Feng and Gu, Zhengquan,, 66, 4269-4275 (2016), doi = https://doi.org/10.1099/ijsem.0.001346, publicationName = Microbiology Society, issn = 1466-5026, abstract= A Gram-staining-negative, non-motile, orange-coloured and rod-shaped aerobic bacterium, designated W18RDT, was isolated from the alpine meadow soil of the Tibetan plateau. Phylogenetic analysis based on 16S rRNA gene sequences positioned strain W18RDT as a representative of a novel species under the genus Sphingomonas which was most closely related to Sphingomonas fennica DSM 13665T with a sequence similarity level of 97.14 %. Meanwhile, it also had a high level of sequence similarity with Sphingomonas laterariae DSM 25432T (96.51 %), Sphingomonas haloaromaticamans CGMCC 1.10206T (96.43 %) and Sphingomonas formosensis DSM 24164T (96.26 %). The G+C content of the genomic DNA of the type strain W18RDT was 66.4mol%. DNA–DNA relatedness for the type strain W18RDT with respect to its closest phylogenetic relative Sphingomonas. fennica DSM 13665Twas 21.54±1.2 %. Major cellular fatty acids in strain W18RDT were C16 : 1 ω7c and/or C16 : 1 ω6c (48.12 %), C18 : 1 ω7c and/or C18 : 1 ω6c (21.98 %) and C14 : 0 2-OH (14.93 %), with ubiquinone-10 (Q-10) as the predominant respiratory quinone. The polar lipid profile of the strain consisted of phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, diphosphatidylglycerol and two unknown lipids. Based on the evidence from a combination of phenotypic, taxonomic and phylogenetic analyses, strain W18RDT represents a novel species of the genus Sphingomonas , for which the name Sphingomonas prati sp. nov. is proposed. The type strain is W18RDT (=CGMCC 1.15645T=DSM 103336T)., language=, type=