RT Journal Article SR Electronic(1) A1 Manandhar, Pooja A1 Zhang, Gengxin A1 Hu, Yilun A1 Lama, Arun A1 Gao, Feng A1 Gu, ZhengquanYR 2016 T1 Sphingomonas prati sp. nov., isolated from alpine meadow soil JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 10 SP 4269 OP 4275 DO https://doi.org/10.1099/ijsem.0.001346 PB Microbiology Society, SN 1466-5034, AB A Gram-staining-negative, non-motile, orange-coloured and rod-shaped aerobic bacterium, designated W18RDT, was isolated from the alpine meadow soil of the Tibetan plateau. Phylogenetic analysis based on 16S rRNA gene sequences positioned strain W18RDT as a representative of a novel species under the genus Sphingomonas which was most closely related to Sphingomonas fennica DSM 13665T with a sequence similarity level of 97.14 %. Meanwhile, it also had a high level of sequence similarity with Sphingomonas laterariae DSM 25432T (96.51 %), Sphingomonas haloaromaticamans CGMCC 1.10206T (96.43 %) and Sphingomonas formosensis DSM 24164T (96.26 %). The G+C content of the genomic DNA of the type strain W18RDT was 66.4mol%. DNA–DNA relatedness for the type strain W18RDT with respect to its closest phylogenetic relative Sphingomonas. fennica DSM 13665Twas 21.54±1.2 %. Major cellular fatty acids in strain W18RDT were C16 : 1 ω7c and/or C16 : 1 ω6c (48.12 %), C18 : 1 ω7c and/or C18 : 1 ω6c (21.98 %) and C14 : 0 2-OH (14.93 %), with ubiquinone-10 (Q-10) as the predominant respiratory quinone. The polar lipid profile of the strain consisted of phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, diphosphatidylglycerol and two unknown lipids. Based on the evidence from a combination of phenotypic, taxonomic and phylogenetic analyses, strain W18RDT represents a novel species of the genus Sphingomonas , for which the name Sphingomonas prati sp. nov. is proposed. The type strain is W18RDT (=CGMCC 1.15645T=DSM 103336T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001346