RT Journal Article SR Electronic(1) A1 Shi, Xu A1 Li, Changfu A1 Zhao, Liang A1 Si, Meiru A1 Zhu, Lingfang A1 Xin, Kaiyun A1 Chen, Chaoqiong A1 Wang, Yao A1 Shen, Xihui A1 Zhang, LeiYR 2016 T1 Rhizobium gei sp. nov., a bacterial endophyte of Geum aleppicum JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 10 SP 4282 OP 4288 DO https://doi.org/10.1099/ijsem.0.001348 PB Microbiology Society, SN 1466-5034, AB A bacterial strain, designated as ZFJT-2T, was isolated from the stem of Geum aleppicum Jacq. collected from Taibai Mountain in Shaanxi Province, north-west China. Cells of strain ZFJT-2T were Gram-stain-negative, strictly aerobic, rod-shaped and motile by means of a single polar flagellum. The major fatty acids were summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c), C16 : 0, 11-methyl C18 : 1 ω7c and summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c), and the DNA G+C content was 58.3 mol% (HPLC). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZFJT-2T was a member of the genus Rhizobium and was most closely related to Rhizobium giardinii KACC 10720T (98.6 % similarity) and Rhizobium herbae CCBAU 83011T (98.5 %). The low levels of sequence similarity found between the atpD, recA and glnII gene sequences of strain ZFJT-2T and those of recognized species of the genus Rhizobium (no more than 94.4, 87.2 and 89.5 %, respectively) indicated that it may represent a separate species of the genus Rhizobium . The DNA–DNA relatedness values for strain ZFJT-2T with respect to R. giardinii KACC 10720T and R. herbae CCBAU 83011T were 17.6 and 41.9 %, respectively. On the basis of phenotypic, phylogenetic and genotypic data, strain ZFJT-2T is considered to represent a novel species of the genus Rhizobium , for which the name Rhizobium gei sp. nov. is proposed. The type strain is ZFJT-2T (=CCTCC AB 2013015T=KCTC 32301T=LMG 27603T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001348