1887

Abstract

A patient strain derived from urine was found by 16S rRNA gene sequencing to be closely related (99.6 % identity) to sequences derived from both CCUG 56047 and CCUG 53855. Those species had been described during the same 11 month period in 2008–2009. Further characterization revealed that those isolates could not be readily distinguished from each other biochemically, by cellular fatty acids, antimicrobial susceptibility, MALDI-TOF MS, 16S rRNA gene sequencing or by whole-genome sequence (WGS) analyses. By WGS comparison, these isolates had an aerage nucleotide identity using n (ANIb) scores of 95.7 % or higher to each other, DNA G+C content in the range of 62.3 mol%–62.4 mol%, with genome sizes ranging from 2.28×10 to 2.41×10 bases. Based on these data, we propose that the name is a later heterotypic synonym of and provide an emended description of .

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2016-11-01
2024-04-19
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References

  1. Bernard K. A., Bellefeuille M., Ewan E. P. 1991; Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic Gram-positive rods. J Clin Microbiol 29:83–89[PubMed]
    [Google Scholar]
  2. Bernard K. A., Munro C., Wiebe D., Ongsansoy E. 2002a; Characteristics of rare or recently described Corynebacterium species recovered from human clinical material in Canada. J Clin Microbiol 40:4375–4381 [View Article][PubMed]
    [Google Scholar]
  3. Bernard K. A., Shuttleworth L., Munro C., Forbes-Faulkner J. C., Pitt D., Norton J. H., Thomas A. D. 2002b; Propionibacterium australiense sp. nov. derived from granulomatous bovine lesions. Anaerobe 8:41–47 [View Article]
    [Google Scholar]
  4. Bernard K. A., Pacheco A. L., Loomer C., Burdz T., Wiebe D., Huynh C., Kaplen B., Olson A. B., Cnockaert M. et al. 2016; Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis. Int J Syst Evol Microbiol 66:2803–2812 [View Article][PubMed]
    [Google Scholar]
  5. Bruker Daltronics 2011 MALDI Biotyper 3.0 User Manual Revision 2 (February 2011) Bremens Germany: Bruker Daltronic gmbH;
    [Google Scholar]
  6. Bruker Daltronics 2014; Bruker Custom MSP and Library Creation. Edited by Anonymous
  7. Clinical Laboratory Standards Institute 2010 M45-2A. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline, 2nd edn. vol. 30 Number 18 Wayne, PA: Clinical Laboratory Standards Institute;
    [Google Scholar]
  8. Euzéby J. P. 2009; List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol 59:1–2 [CrossRef]
    [Google Scholar]
  9. Funke G., Carlotti A. 1994; Differentiation of Brevibacterium spp. encountered in clinical specimens. J Clin Microbiol 32:1729–1732[PubMed]
    [Google Scholar]
  10. Mages I. S., Frodl R., Bernard K. A., Funke G. 2008; Identities of Arthrobacter spp. and Arthrobacter-like bacteria encountered in human clinical specimens. J Clin Microbiol 46:2980–2986 [View Article][PubMed]
    [Google Scholar]
  11. Parker C. T., Tindall B. J., Garrity G. M. 2015; International code of nomenclature of prokaryotes. Int J Syst Evol Microbiol in press, doi: [View Article][PubMed]
    [Google Scholar]
  12. Richter M., Rosselló-Móra R. 2009; Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  13. Richter M., Rosselló-Móra R., Oliver Glöckner F., Peplies J. 2016; JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 32:929–931 [View Article][PubMed]
    [Google Scholar]
  14. Roux V., Raoult D. 2009; Brevibacterium massiliense sp. nov., isolated from a human ankle discharge. Int J Syst Evol Microbiol 59:1960–1964 [View Article][PubMed]
    [Google Scholar]
  15. Roux V., Robert C., Gimenez G., Raoult D. 2012; Draft genome sequence of Brevibacterium massiliense strain 541308T. J Bacteriol 194:5151–5152 [View Article][PubMed]
    [Google Scholar]
  16. Seemann T. 2014; Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068–2069 [View Article][PubMed]
    [Google Scholar]
  17. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  18. Trujillo M. E., Goodfellow M. 2012; Genus I. Brevibacterium Breed 1953a. 13AL emend. Colins, Jones, Keddie and Sneath 1980. In Bergeys Manual of Systematic Bacteriology, 2nd edn. vol. 5: The Actinobacteria , pp. 685–700 . Edited by Whitman W. B., Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M., Ludwig W., Suzuki K.-i., Parte A. New York: Springer; [CrossRef]
    [Google Scholar]
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