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Volume 66, Issue 11

sp. nov., isolated from chickens with spotty liver disease Free

Abstract

Ten strains of an unknown species were isolated from the livers of chickens with spotty liver disease in Australia. The strains were Gram-stain-negative, microaerobic, catalase- and oxidase-positive and urease-negative. Unlike most other species of the genus , most of the tested strains of this novel species hydrolysed hippurate and half of them could not reduce nitrate. All strains showed resistance, or intermediate resistance, to nalidixic acid and most of them were resistant to cephalothin. Examination of negatively stained cells under transmission electron microscopy revealed that they were S-shaped, with bipolar unsheathed flagella. Phylogenetic analyses based on the 16S rRNA gene and the heat shock protein 60 () gene sequences indicated that the strains formed a robust clade that was clearly distinct from recognized species. Unusually, they had a DNA G+C content of 27.9 mol%, lower than any previously described species, and they showed less than 84 % average nucleotide identity to the nearest sequenced species. Taken together, these data indicate that the strains belong to a novel species, for which the name sp. nov. is proposed. The type strain is HV10 (=NCTC 13823=CIP 111092).

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  • Accepted:
  • Published Online:
Keyword(s): bipolar flagella , Campylobacter hepaticus , chicken , low G+C and spotty liver disease
© 2016 IUMS
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2016-11-01
2024-04-20
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Campylobacter hepaticus sp. nov., isolated from chickens with spotty liver disease
Int J Syst Evol Microbiol 66, 4518 (2016); https://doi.org/10.1099/ijsem.0.001383
/content/journal/ijsem/10.1099/ijsem.0.001383
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