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Volume 66, Issue 11

sp. nov., isolated from wetland freshwater Free

Abstract

A non-motile, rod-shaped and pale-pink bacterium, designated strain WS71, was isolated from freshwater collected from the Woopo Wetland (Republic of Korea). Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were summed feature 3 (comprising Cω6 and/or Cω7), iso-C 3-OH, C and iso-C. The strain contained menaquinone 7 (MK-7) and the DNA G+C content was 39.4±0.4 mol%. The major polar lipids were phosphatidylethanolamine and an unknown aminophospholipid. A phylogenetic tree based on 16S rRNA gene sequences showed that WS71 forms an evolutionary lineage within the radiation enclosing the members of genus with HME6664 as its nearest neighbor (98.3 % sequence similarity). DNA–DNA relatedness between WS71 and HME6664 was 61.3±1.0 %. A number of phenotypic characteristics distinguished WS71 from the other members of the genus . On the basis of the evidence presented in this study, WS71 represents a novel species, for which the name sp. nov. is proposed. The type strain is WS71 (=KCTC 32270=JCM 19495).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Mucilaginibacter roseus and wetland freshwater
© 2016 IUMS
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2016-11-01
2024-04-24
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References

  1. An D. S., Yin C. R., Lee S. T., Cho C. H. 2009; Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. Int J Syst Evol Microbiol 59:1122–1125 [View Article][PubMed]
     [Google Scholar]
  2. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N. 2010; Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol 60:134–139 [View Article][PubMed]
     [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  4. Chen X. Y., Zhao R., Tian Y., Kong B. H., Li X. D., Chen Z. L., Li Y. H. 2014; Mucilaginibacter polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol 64:1395–1400 [View Article][PubMed]
     [Google Scholar]
  5. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
     [Google Scholar]
  6. CLSI 2009 Performance Standards for Antimicrobial Susceptibility Testing 19th Informational Supplement. CLSI Document (ISBN 1-56238-690-5) M100–S19 Wayne, PA: Clinical and Laboratory Standards Institute;
     [Google Scholar]
  7. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp 265–309 Edited by Goodfellow M., O’Donnell A. G. Chichester: Johb Wiley & Sons Ltd;
     [Google Scholar]
  8. Cui C. H., Choi T. E., Yu H., Jin F., Lee S. T., Kim S. C., Im W. T. 2011; Mucilaginibacter composti sp. nov., with ginsenoside converting activity, isolated from compost. J Microbiol 49:393–398 [View Article][PubMed]
     [Google Scholar]
  9. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Chemical Methods in Prokaryotic Systematics pp 121–161 Edited by Goodfellow M., O’Donell A. G. Chichester: Wiley;
     [Google Scholar]
  10. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  11. Felsenstein J. 1993 PHYLIP (phylogeny inference package), version 3.5c., Distributed by the author. Department of Genome Sciences University of Washington, Seattle, USA
  12. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  13. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
     [Google Scholar]
  14. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98
     [Google Scholar]
  15. Hwang Y. M., Baik K. S., Seong C. N. 2014; Mucilaginibacter defluvii sp. nov., isolated from a dye wastewater treatment facility. Int J Syst Evol Microbiol 64:565–571 [View Article][PubMed]
     [Google Scholar]
  16. Jiang F., Dai J., Wang Y., Xue X., Xu M., Guo Y., Li W., Fang C., Peng F. 2012; Mucilaginibacter soli sp. nov., isolated from Arctic tundra soil. Int J Syst Evol Microbiol 62:1630–1635 [View Article][PubMed]
     [Google Scholar]
  17. Joung Y., Kim H., Kang H., Lee B., Ahn T. S., Joh K. 2014; Mucilaginibacter soyangensis sp. nov., isolated from a lake. Int J Syst Evol Microbiol 64:413–419 [View Article][PubMed]
     [Google Scholar]
  18. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 , pp 21–132 Edited by Munro H. N. New York: Academic Press; [CrossRef]
     [Google Scholar]
  19. Khan H., Chung E. J., Kang D. Y., Jeon C. O., Chung Y. R. 2013; Mucilaginibacter jinjuensis sp. nov., with xylan-degrading activity. Int J Syst Evol Microbiol 63:1267–1272 [View Article][PubMed]
     [Google Scholar]
  20. Kim B. C., Lee K. H., Kim M. N., Lee J., Shin K. S. 2010; Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum. FEMS Microbiol Lett 309:130–135 [View Article][PubMed]
     [Google Scholar]
  21. Kim J. H., Kang S. J., Jung Y. T., Oh T. K., Yoon J. H. 2012a; Mucilaginibacter lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 62:515–519 [View Article][PubMed]
     [Google Scholar]
  22. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012b; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  23. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article][PubMed]
     [Google Scholar]
  24. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
     [Google Scholar]
  25. Lee J. S., Lee K. C., Pyun Y. R., Bae K. S. 2003; Arthrobacter koreensis sp. nov., a novel alkalitolerant bacterium from soil. Int J Syst Evol Microbiol 53:1277–1280 [View Article][PubMed]
     [Google Scholar]
  26. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C. 2009; Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. Int J Syst Evol Microbiol 59:1447–1450 [View Article][PubMed]
     [Google Scholar]
  27. Madhaiyan M., Poonguzhali S., Lee J. S., Senthilkumar M., Lee K. C., Sundaram S. 2010; Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. Int J Syst Evol Microbiol 60:2451–2457 [View Article][PubMed]
     [Google Scholar]
  28. Männistö M. K., Tiirola M., McConnell J., Häggblom M. M. 2010; Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. Int J Syst Evol Microbiol 60:2849–2856 [View Article][PubMed]
     [Google Scholar]
  29. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
     [Google Scholar]
  30. Minnikin D. E., Patel P. V, Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
     [Google Scholar]
  31. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  32. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N. 2007; Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol 57:2349–2354 [View Article][PubMed]
     [Google Scholar]
  33. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  34. Smibert R. M., Krieg N. R. 1994; General characterization. In Methods for General and Molecular Bacteriology pp 607–654 Edited by Gebhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  35. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48:165–177 [View Article][PubMed]
     [Google Scholar]
  36. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  37. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  38. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology Press;
     [Google Scholar]
  39. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M. 2008; Mucilaginibacter kameinonensis sp. nov., isolated from garden soil. Int J Syst Evol Microbiol 58:2046–2050 [CrossRef]
     [Google Scholar]
  40. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  41. Yoon J. H., Kang S. J., Park S., Oh T. K. 2012; Mucilaginibacter litoreus sp. nov., isolated from marine sand. Int J Syst Evol Microbiol 62:2822–2827 [View Article][PubMed]
     [Google Scholar]
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Mucilaginibacterpuniceus sp. nov., isolated from wetland freshwater
Int J Syst Evol Microbiol 66, 4549 (2016); https://doi.org/10.1099/ijsem.0.001389
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