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f Marinifilum albidiflavum sp. nov., isolated from coastal sediment

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Marinifilum albidiflavum sp. nov., isolated from coastal sediment, Page 1 of 1

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  • A novel Gram-stain-negative, facultatively anaerobic, filamentous, and yellowish-white-pigmented marine bacterium, designated strain FB208, was isolated from marine sediment obtained off the coastal area of Weihai, China. Cells of strain FB208 were filamentous during exponential growth, fragmented to rods in the stationary growth phase and became spherical in aged cultures. It grew optimally at 33 °C, at pH 7.0–7.5 and in the presence of 2.0–3.0 % (w/v) NaCl. Based on the 16S rRNA gene sequence, strain FB208 was found to be closely related to Marinifilum flexuosum DSM 21950 (96.9 % similarity) and Marinifilum fragile JCM 15579 (96.4 %), with less than 90.0 % sequence similarity to other genera of the class Bacteroidia . Phylogenetic analysis, also based on 16S rRNA gene sequences, placed strain FB208 in the genus Marinifilum , family Marinifilaceae . The predominant isoprenoid quinone of strain FB208 was identified as menaquinone MK-7. The main cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C17 : 1 ω9c, and the major polar lipids were an unidentified lipid and aminophospholipid. The G+C content of the genomic DNA was 43.8 mol%. Based on these phylogenetic and phenotypic data, strain FB208 represents a novel species of the genus Marinifilum , for which the name Marinifilum albidiflavum sp. nov. is proposed. The type strain is FB208 (=KCTC 42591=MCCC 1H00113).

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Marinifilum albidiflavum FB208 is KX010419.

  • Two supplementary figures are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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2016-11-01
2019-02-23
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