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- Volume 66, Issue 11
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f Nocardia jiangsuensis sp. nov., an actinomycete isolated from coastal soil
- Authors: Juan-Luan Bai1,† , Yu Wang1,† , Sheng Qin1 , Peng Ding1 , Ke Xing1 , Bo Yuan1 , Cheng-Liang Cao1 , Ying Huang2 , Yu-Qin Zhang3 , Ji-Hong Jiang1
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- VIEW AFFILIATIONS
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1 1School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China 2 2State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China 3 3Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China
- Correspondence Sheng Qin [email protected]
- First Published Online: 01 November 2016, International Journal of Systematic and Evolutionary Microbiology 66: 4633-4638, doi: 10.1099/ijsem.0.001402
- Subject: New taxa - Actinobacteria
- Received:
- Accepted:
- Cover date:




Nocardia jiangsuensis sp. nov., an actinomycete isolated from coastal soil, Page 1 of 1
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A novel actinomycete strain, designated strain KLBMP S0027T, was isolated from a coastal soil collected from the coastal region of Lianyungang, Jiangsu Province, in east China, and was studied in detail for its taxonomic position. Comparative 16S rRNA gene sequence analysis showed that this strain belonged to the genus Nocardia and was most closely related to Nocardia harenae WS-26T (98.5 %), Nocardia asiatica NBRC 100129T (98.5 %), Nocardia abscessus NBRC 100374T (98.2 %), Nocardia brasiliensis NBRC 14402T (98.2 %) and Nocardia cyriacigeorgica NBRC 100375T (98 %), respectively. The strain showed a combination of chemotaxonomic and morphological properties typical of the genus Nocardia . The cell wall contained meso-diaminopimelic acid (type IV), and whole-cell sugars were arabinose, galactose, glucose and ribose. Strain KLBMP S0027T contained MK-8(H4ω-cycl) as the predominant menaquinone; C16 : 0, C18 : 1ω9c, C18 : 0 10-methyl (TBSA) and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) were the major cellular fatty acids. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, one unknown glycolipid and two unknown lipids. Mycolic acids were detected. The G+C content of the DNA was 70.5 %. However, a combination of DNA–DNA hybridization and phenotypic data demonstrated that strain KLBMP S0027T could be clearly distinguished from the type strain of the most closely related species, N. harenae WS-26T. On the basis of the data presented from a polyphasic study, it was evident that this strain should be assigned to a novel species of the genus Nocardia , for which the name Nocardia jiangsuensis sp. nov. is proposed. The type strain is KLBMP S0027T (=CGMCC 4.7330T=KCTC 39691T).
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†
These authors contributed equally to this work.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KLBMP S0027T is KP972626.
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Six supplementary figures are available with the online Supplementary Material.
- Keyword(s): Actinomycetes, Novel species, Nocardia, 16S rRNA gene, Polyphasic taxonomy
© 2016 IUMS | Published by the Microbiology Society
-
Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E..( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol100:221––230. [CrossRef][PubMed]
-
Ezaki T., Hashimoto Y., Yabuuchi E..( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol39:224––229. [CrossRef]
-
Ezeoke I., Klenk H. P., Pötter G., Schumann P., Moser B. D., Lasker B. A., Nicholson A., Brown J. M..( 2013;). Nocardia amikacinitolerans sp. nov., an amikacin-resistant human pathogen. . Int J Syst Evol Microbiol63:1056––1061. [CrossRef][PubMed]
-
Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol17:368––376. [CrossRef][PubMed]
-
Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution39:783––791. [CrossRef]
-
Goodfellow M..( 1971;). Numerical taxonomy of some nocardioform bacteria. . J Gen Microbiol69:33––80. [CrossRef][PubMed]
-
Goodfellow M., Lechevalier M. P..( 1989;). Genus Nocardia trevisan 1889, 9AL. . In Bergey’s Manual of Systematic Bacteriology,vol. 4 pp. 2348––2361. Edited by Williams S. T., Sharpe M. E., Holt J. G.. Baltimore:: Williams & Wilkins;.
-
Goodfellow M., Jones A. L..( 2012;). Order V. Corynebacteriales ord. nov. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 5 pp. 232––243. Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B.. New York:: Springer;.[CrossRef]
-
Goodfellow M., Isik K., Yates E..( 1999;). Actinomycete systematics: an unfinished synthesis. . Nova Acta Leopold312:47––82.
-
Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H..( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol24:54––63. [CrossRef]
-
Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K..( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol47:1129––1133. [CrossRef][PubMed]
-
He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J..( 2005;). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. . Int J Syst Evol Microbiol55:1939––1944. [CrossRef][PubMed]
-
Kaewkla O., Franco C. M..( 2010;). Nocardia callitridis sp. nov., an endophytic actinobacterium isolated from a surface-sterilized root of an Australian native pine tree. . Int J Syst Evol Microbiol60:1532––1536. [CrossRef][PubMed]
-
Kelly K. L..( 1964;). Inter-Society Color Council-National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors published in US. Washington, DC:: US Government Printing;.
-
Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol62:716––721. [CrossRef][PubMed]
-
Kim M., Oh H. S., Park S. C., Chun J..( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. . Int J Syst Evol Microbiol64:346––351. [CrossRef][PubMed]
-
Kluge A. G., Farris J. S..( 1969;). Quantitative phyletics and the evolution of anurans. . Syst Zool18:1––32. [CrossRef]
-
Lasker B. A., Bell M., Klenk H. P., Spröer C., Schumann C., Schumann P., Brown J. M..( 2014;). Nocardia vulneris sp. nov., isolated from wounds of human patients in North America. . Antonie Van Leeuwenhoek106:543––553. [CrossRef][PubMed]
-
Lechevalier M. P., Lechevalier H..( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol20:435––443. [CrossRef]
-
Mesbah M., Premachandran U., Whitman W. B..( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol39:159––167. [CrossRef]
-
Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M..( 1980;). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr188:221––233. [CrossRef]
-
Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H..( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2:233––241. [CrossRef]
-
Qin S., Chen H. H., Zhao G. Z., Li J., Zhu W. Y., Xu L. H., Jiang J. H., Li W. J..( 2012;). Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austroyunnanensis in Xishuangbanna tropical rainforest revealed by culture-dependent and culture-independent methods. . Environ Microbiol Rep4:522––531. [CrossRef][PubMed]
-
Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol4:406––425.[PubMed]
-
Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, , MIDI Technical Note 101.. Newark, DE:: MIDI Inc;.
-
Sazak A., Sahin N., Camas M..( 2012;). Nocardia goodfellowii sp. nov. and Nocardia thraciensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol62:1228––1234. [CrossRef][PubMed]
-
Seo J. P., Lee S. D..( 2006;). Nocardia harenae sp. nov., an actinomycete isolated from beach sand. . Int J Syst Evol Microbiol56:2203––2207. [CrossRef][PubMed]
-
Shirling E. B., Gottlieb D..( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol16:313––340. [CrossRef]
-
Stackebrandt E., Ebers J..( 2006;). Taxonomic parameters revisited: tarnished gold standards. . Microbiology Today33:152––155.
-
Takeda K., Kang Y., Yazawa K., Gonoi T., Mikami Y..( 2010;). Phylogenetic studies of Nocardia species based on gyrB gene analyses. . J Med Microbiol59:165––171. [CrossRef][PubMed]
-
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S..( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol28:2731––2739. [CrossRef][PubMed]
-
Tanasupawat S., Phongsopitanun W., Suwanborirux K., Ohkuma M., Kudo T..( 2016;). Nocardia rayongensis sp. nov., isolated from Thai peat swamp forest soil. . Int J Syst Evol Microbiol66:1950––1955. [CrossRef][PubMed]
-
Trevisan V..( 1889;). I Generi E Le Specie Delle Batteriacee. Milan:: Zanaboni & Gabuzzi;.
-
Vaddavalli R., Peddi S., Kothagauni S. Y., Linga V. R..( 2014;). Nocardia bhagyanesis sp. nov., a novel actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis), India. . Antonie Van Leeuwenhoek105:443––450. [CrossRef][PubMed]
-
Waksman S. A..( 1967;). The Actinomycetes. A Summary of Current Knowledge. New York:: Ronald Press;.
-
Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E. et al.( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37:463––464.[CrossRef]
-
Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J..( 1983;). Numerical classification of Streptomyces and related genera. . J Gen Microbiol129:1743––1813. [CrossRef][PubMed]
-
Xing K., Qin S., Fei S. M., Lin Q., Bian G. K., Miao Q., Wang Y., Cao C. L., Tang S. K. et al.( 2011;). Nocardia endophytica sp. nov., an endophytic actinomycete isolated from the oil-seed plant Jatropha curcas L. . Int J Syst Evol Microbiol61:1854––1858. [CrossRef][PubMed]
-
Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L..( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae' isolated from China. . Int J Syst Evol Microbiol55:1149––1153. [CrossRef][PubMed]

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